{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:31:40Z","timestamp":1787063500785,"version":"build-2736575974"},"reference-count":39,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Otolaryngology - Head &amp; Neck Surgery"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec>\n                    <jats:title>Importance<\/jats:title>\n                    <jats:p>Nasopharyngeal carcinoma (NPC) is closely linked to microorganisms, especially intra-tumoral microbiota. However, the role of commensal microbiota in NPC remains underexplored, with implications for understanding disease mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aims to analyze and compare the bacterial microbiota in the nasopharynx and middle meatus (MM) of individuals with NPC and those without NPC. Additionally, the study seeks to identify potential microbial biomarkers that can distinguish between NPC and non-NPC (nNPC) individuals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>Cross-sectional study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>Study conducted in a clinical setting with NPC and non-NPC participants to evaluate microbial diversity relevant to NPC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Participants<\/jats:title>\n                    <jats:p>Ten NPC cases and 15 non-NPC controls were recruited based on clinical eligibility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main Outcome Measures<\/jats:title>\n                    <jats:p>Bacterial microbiota sampling from the nasopharynx and MM was analyzed by 16S rRNA sequencing. Microbiota diversity (alpha and beta diversity indices), presence of bacterial taxa with biomarker potential, and prediction model accuracy [area under the curve (AUC)].<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Microbiota diversity was significantly lower in NPC patients versus controls. In nasopharyngeal samples, alpha diversity (Chao1 index, P\u2009=\u2009.02) and beta diversity (PERMANOVA, P\u2009=\u2009.001) differed notably between groups, though MM samples showed no significant difference (Chao1 index, P\u2009=\u2009.23). Machine learning identified Pseudomonas, Cutibacterium, and Finegoldia as potential NPC biomarkers (AUC\u2009=\u20090.86).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Relevance<\/jats:title>\n                    <jats:p>This pioneering study highlights dysbiosis in nasopharyngeal microbiota among NPC patients. Findings suggest that Pseudomonas, Cutibacterium, and Finegoldia may be useful biomarkers for NPC diagnosis, warranting further investigation into microbial roles in NPC pathogenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19160216241304365","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T06:30:44Z","timestamp":1734676244000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Microbial Dysbiosis in Nasopharyngeal Carcinoma: A Pilot Study on Biomarker Potential"],"prefix":"10.1177","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7034-4149","authenticated-orcid":false,"given":"Yen-Ting","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, St. Martin De Porres Hospital, Chiayi, Taiwan"},{"name":"Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan"},{"name":"School of Medicine, Chung Shan Medical University, Taichung, Taiwan"},{"name":"Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Han","family":"Hsin","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan"},{"name":"School of Medicine, Chung Shan Medical University, Taichung, Taiwan"},{"name":"Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Yi","family":"Chuang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan"},{"name":"School of Medicine, Chung Shan Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Chen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan"},{"name":"School of Medicine, Chung Shan Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao-Chang","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan"},{"name":"School of Medicine, Chung Shan Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Sheng","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan"},{"name":"School of Medicine, Chung Shan Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Hung","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yih-Yuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Yang","family":"Lee","sequence":"additional","affiliation":[{"name":"Office of Information Technology, Taipei Medical University, Taipei City, Taiwan"},{"name":"Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University, Taipei City, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Xuan","family":"Li","sequence":"additional","affiliation":[{"name":"Office of Information Technology, Taipei Medical University, Taipei City, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Chou","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, St. Martin De Porres Hospital, Chiayi, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Hao","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University, Taipei City, Taiwan"},{"name":"Clinical Big Data Research Center, Taipei Medical University Hospital, Taipei City, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun-Fa","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan"},{"name":"Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"bibr1-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2022.2810"},{"issue":"16","key":"bibr2-19160216241304365","first-page":"5104","volume":"25","author":"Song P","year":"2021","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"bibr3-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-008-9661-9"},{"key":"bibr4-19160216241304365","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.859721"},{"key":"bibr5-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1002\/cre2.139"},{"key":"bibr6-19160216241304365","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21093044"},{"key":"bibr7-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1080\/07357907.2020.1732405"},{"key":"bibr8-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)30956-0"},{"key":"bibr9-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1016\/S1044579X02000858"},{"key":"bibr10-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23215"},{"key":"bibr11-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1186\/s40463-020-00482-x"},{"key":"bibr12-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204568"},{"key":"bibr13-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-022-01449-2"},{"key":"bibr14-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22741"},{"key":"bibr15-19160216241304365","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.1013920"},{"key":"bibr16-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-24921-z"},{"key":"bibr17-19160216241304365","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8111973"},{"key":"bibr18-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42548-5"},{"key":"bibr19-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0548-6"},{"key":"bibr20-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu494"},{"key":"bibr21-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21871"},{"key":"bibr22-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000860"},{"key":"bibr23-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02079-3"},{"key":"bibr24-19160216241304365","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00115"},{"key":"bibr25-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1392-3"},{"key":"bibr26-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12033"},{"key":"bibr27-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041772"},{"key":"bibr28-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-44460-4"},{"key":"bibr29-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-82465-0"},{"key":"bibr30-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1016\/j.ciresp.2010.08.006"},{"key":"bibr31-19160216241304365","doi-asserted-by":"publisher","DOI":"10.3390\/biology10060533"},{"key":"bibr32-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.58"},{"key":"bibr33-19160216241304365","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12061447"},{"key":"bibr34-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2004.02.005"},{"key":"bibr35-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2024.106864"},{"key":"bibr36-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.10.014"},{"key":"bibr37-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201808325"},{"key":"bibr38-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605007"},{"key":"bibr39-19160216241304365","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2008.05.005"}],"container-title":["Journal of Otolaryngology - Head &amp; Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/19160216241304365","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/19160216241304365","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/19160216241304365","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:53:24Z","timestamp":1777589604000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/19160216241304365"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":39,"alternative-id":["10.1177\/19160216241304365"],"URL":"https:\/\/doi.org\/10.1177\/19160216241304365","relation":{},"ISSN":["1916-0216","1916-0216"],"issn-type":[{"value":"1916-0216","type":"print"},{"value":"1916-0216","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"article-number":"19160216241304365"}}