{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:52:00Z","timestamp":1777877520836,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Dr Azar-Angelil Research Chair in Head and Neck Oncology of the Universit\u00e9 de Montr\u00e9al"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Otolaryngology - Head &amp; Neck Surgery"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Importance<\/jats:title>\n                    <jats:p>The inclusion of depth of invasion (DOI) in the American Joint Committee on Cancer\u2019s staging system for oral tongue squamous cell carcinoma (OTSCC) has major clinical implications. Few studies have evaluated the accuracy of preoperative biopsy to predict DOI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the reliability of preoperative punch biopsy for measuring DOI in early OTSCC and compare it to evaluation by digital palpation. Secondarily, to assess the punch biopsy\u2019s ability to differentiate between carcinoma in situ (Tis) and invasive carcinoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>A prospective single-center cohort study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>Center Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al, a tertiary center in Canada.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Participants<\/jats:title>\n                    <jats:p>Patients with suspected early stage OTSCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Intervention<\/jats:title>\n                    <jats:p>Punch biopsy was used to sample the deepest part of tumors to measure biopsy-derived DOI (bDOI). In addition, DOI was estimated via digital palpation: clinical DOI (cDOI) by surgeons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main Outcome Measures<\/jats:title>\n                    <jats:p>Pathologic DOI (pDOI) from final histopathology reports was the gold standard. Spearman\u2019s correlations were calculated between cDOI, bDOI, and pDOI. Diagnostic performance metrics were calculated for the ability to distinguish pDOI of \u22652\u2009mm, and to differentiate Tis from invasive carcinoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among 27 patients, correlation coefficients between bDOI and pDOI, and cDOI and pDOI were 0.603 (95% CI: 0.202-0.884) and 0.894 (95% CI: 0.749-0.955), respectively. Punch biopsy sensitivity and specificity were 0.88 (95% CI: 0.62-0.98) and 0.91 (95% CI: 0.59-0.99) to detect pDOI\u2009\u2265\u20092\u2009mm, and 0.89 (95% CI: 0.65-0.99) and 0.86 (95% CI: 0.42-1.00) for distinguishing Tis from invasive carcinoma. Digital palpation sensitivity and specificity for pDOI\u2009\u2265\u20092\u2009mm were 0.86 (95% CI: 0.57-0.98) and 1.00 (95% CI: 0.63-1.00).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Punch biopsy and clinical palpation demonstrate high diagnostic yield for identifying lesions with pDOI\u2009\u2265\u20092\u2009mm. Punch biopsy appears to be reliable to distinguish Tis from invasive carcinoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Relevance<\/jats:title>\n                    <jats:p>Larger studies are needed to corroborate these findings and assess the role of punch biopsy in guiding elective neck dissection decisions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19160216251321452","type":"journal-article","created":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T02:34:13Z","timestamp":1743561253000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Punch Biopsy for Preoperative Depth of Invasion Assessment in Early Oral Tongue Squamous Cell Carcinoma: A Prospective Pilot Study"],"prefix":"10.1177","volume":"54","author":[{"given":"B\u00e9atrice","family":"Voizard","sequence":"first","affiliation":[{"name":"Division of Otolaryngology 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Universit\u00e9 de Montr\u00e9al, Montreal, QC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis","family":"Guertin","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology \u2013 Head and Neck Surgery, Department of Surgery, Universit\u00e9 de Montr\u00e9al, Montreal, QC, Canada"},{"name":"Division of Otolaryngology \u2013 Head and Neck Surgery, Center Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al, Universit\u00e9 de Montr\u00e9al, Montreal, QC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Tabet","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology \u2013 Head and Neck Surgery, Department of Surgery, Universit\u00e9 de Montr\u00e9al, Montreal, QC, Canada"},{"name":"Division of Otolaryngology \u2013 Head and Neck Surgery, Center Hospitalier de l\u2019Universit\u00e9 de Montr\u00e9al, Universit\u00e9 de Montr\u00e9al, Montreal, QC, 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