{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:10:13Z","timestamp":1786122613732,"version":"build-2736575974"},"reference-count":28,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Otolaryngology - Head &amp; Neck Surgery"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Importance:<\/jats:title>\n                    <jats:p>Accurate HPV status diagnosis is essential for managing oropharyngeal squamous cell carcinoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>We aimed to refine HPV status determination using a cost-effective and reliable approach by integrating the p53 immunohistochemical marker with the conventional p16 marker. We assessed the association between HPV status and the p16\/p53 tumor immunohistochemical status and analyzed patient survival based on p16\/p53 expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design, Setting, Participants, and Exposures:<\/jats:title>\n                    <jats:p>This retrospective, single-center study included 285 patients with oropharyngeal squamous cell carcinoma (2017-2022) with known p16\/p53 immunohistochemical profiles. In a subset of 92 patients, HPV status was formally determined using polymerase chain reaction or in situ hybridization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Outcome Measures and Results:<\/jats:title>\n                    <jats:p>\n                      The p16\/p53 test was an excellent predictor of HPV status (specificity: 100%, sensitivity: 98.48%), significantly outperforming p16 alone (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009&lt;\u2009.01). Patients with a p16+\/p53WT profile had superior 5-year overall survival (82.2%; 108\/131) and recurrence-free survival (79.4%; 104\/131). Patients with discordant profiles (p16+\/p53MT, p16\u2212\/p53WT) had survival outcomes similar to HPV-independent oropharyngeal squamous cell carcinomas.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The p16\/p53 immunohistochemical profile appears to be a reliable surrogate marker for HPV status and a strong predictor of survival. It could help identify discordant cases, helping prevent inappropriate therapeutic de-escalation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Relevance:<\/jats:title>\n                    <jats:p>With the advancement of artificial intelligence, integrating additional markers into an algorithmic analysis could further refine patient classification and enable more personalized therapeutic approaches.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19160216261451818","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T09:14:13Z","timestamp":1785834853000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Prognostic Value of the P16\/P53 Immunohistochemical Association in Oropharyngeal Squamous Cell Carcinomas"],"prefix":"10.1177","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1027-0637","authenticated-orcid":false,"given":"Antoine","family":"Lagadec","sequence":"first","affiliation":[{"name":"Service d\u2019Oto-Rhino-Laryngologie et Chirurgie Cervico-Faciale, H\u00f4pital La Croix Rousse, Hospices Civils de Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazim","family":"Benzerdjeb","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institut de Pathologie Multisite, Groupement Hospitalier Sud, Hospices Civils de Lyon, Pierre-B\u00e9nite, France"},{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 Lyon 1, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Lapierre","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 Lyon 1, France"},{"name":"Service de Radioth\u00e9rapie, Groupement Hospitalier Sud, Hospices Civil de Lyon, Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyad","family":"Alsugair","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institut de Pathologie Multisite, Groupement Hospitalier Sud, Hospices Civils de Lyon, Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Bruyas","sequence":"additional","affiliation":[{"name":"Service d\u2019Oncologie, H\u00f4pital La Croix Rousse, Hospices Civils de Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Blanc","sequence":"additional","affiliation":[{"name":"Service d\u2019Oto-Rhino-Laryngologie et Chirurgie Cervico-Faciale, H\u00f4pital La Croix Rousse, Hospices Civils de Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"C\u00e9ruse","sequence":"additional","affiliation":[{"name":"Service d\u2019Oto-Rhino-Laryngologie et Chirurgie 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