{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T11:51:20Z","timestamp":1782301880103,"version":"3.54.5"},"reference-count":30,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2025,7,1]]},"abstract":"<jats:p>\n                    Bioengineering aims to develop biomaterials that closely mimic the native extracellular matrix (ECM) to support tissue regeneration. This study presents a detailed protocol for producing hydrogels derived from decellularized bovine placental cotyledons. Bovine placentas at 4\u20135 months of gestation (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 10) were subjected to vascular perfusion with increasing concentrations of sodium dodecyl sulfate (0.01\u20131%) and Triton X-100 (1%), which effectively removed cellular components. Decellularization efficacy was confirmed by histological (hematoxylin and eosin and 4\u2032,6-diamidino-2-phenylindole [DAPI] staining), molecular, and structural analyses, including residual genomic DNA quantification averaging 9.1 ng\/mg of dry tissue. The ECM scaffolds were enzymatically digested using 0.1% (w\/v) pepsin in 0.01 M HCl and reconstituted with sodium alginate at concentrations of 5%, 8%, 10%, and 12% (w\/v). Crosslinking was achieved with 1% calcium chloride. Among the tested formulations, hydrogels containing 12% alginate demonstrated greater mechanical stability and preserved three-dimensional architecture, including interconnected porosity, as evidenced by scanning electron microscopy. Cytocompatibility was evaluated by culturing canine adipose-derived mesenchymal stem cells on both decellularized biomaterials and hydrogels. DAPI staining revealed nuclei after 7 and 25 days of culture, indicating cell presence and distribution throughout the constructs. These results indicate that bovine cotyledon-derived ECM hydrogels maintain structural and biochemical features favorable for cell interaction and may serve as adaptable platforms for tissue engineering, dermal repair, and three-dimensional cell culture.\n                  <\/jats:p>","DOI":"10.1177\/19373341251360957","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T06:59:13Z","timestamp":1759820353000},"page":"271-279","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Bovine Placental Cotyledon-Derived Hydrogel: Methodology to Produce a Substrate for Tissue Engineering"],"prefix":"10.1177","volume":"31","author":[{"given":"Ra\u00ed Andr\u00e9 Querino","family":"Candel\u00e1ria","sequence":"first","affiliation":[{"name":"Regenerative and Reproductive Biotechnology Laboratory, Department of Animal Morphology and Physiology, School of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University (UNESP), Jaboticabal, Brazil."},{"name":"Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (USP), S\u00e3o Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor S.","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (USP), S\u00e3o Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ang\u00e9lica","family":"Miglino","sequence":"additional","affiliation":[{"name":"Graduation Program in Structural and Functional Interactions in Rehabilitation, Department of Postgraduate Studies, University of Mar\u00edlia (UNIMAR), Mar\u00edlia, Brazil."},{"name":"Graduation Program in Animal Health, Production and Environment, University of Mar\u00edlia (UNIMAR), Mar\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5240-6959","authenticated-orcid":false,"given":"Rodrigo S.N.","family":"Barreto","sequence":"additional","affiliation":[{"name":"Regenerative and Reproductive Biotechnology Laboratory, Department of Animal Morphology and Physiology, School of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University (UNESP), Jaboticabal, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.01.057"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-7021(11)70058-X"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/jfb13040240"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9831534"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/term.2618"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.36660\/abc.20220816"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000492466"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.09.035"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1201\/9781420027563"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-018-0023-x"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.04.188"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1992.tb01940.x"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jceh.2014.03.043"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470559277.ch140272"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.04.003"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0714"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0602"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201705739"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.12.014"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioadv.2022.212806"},{"key":"e_1_3_6_22_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2009.0639"},{"key":"e_1_3_6_23_2","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics16030372"},{"key":"e_1_3_6_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez139"},{"key":"e_1_3_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtcomm.2022.104478"},{"key":"e_1_3_6_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-82409-4"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031530"},{"key":"e_1_3_6_28_2","doi-asserted-by":"publisher","DOI":"10.1637\/0005-2086(2007)51[555:TMGSRI]2.0.CO;2"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/polym15214228"},{"key":"e_1_3_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125672"},{"key":"e_1_3_6_31_2","doi-asserted-by":"publisher","DOI":"10.31083\/j.fbl2906228"}],"container-title":["Tissue 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