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Organoids exhibit organ-specific architecture, function, and multicellular composition, can be infinitely derived from pluripotent stem cells, and can be further directed toward organoids of the endocrine or exocrine pancreas. Pancreatic endocrine organoids are rapidly redefining diabetes therapies due to their ability to recapitulate glucose-responsive insulin secretion. Conversely, there is less focus on pancreatic exocrine organoids, which possess untapped potential for investigating disorders such as cancer and cystic fibrosis. This review first summarizes human pancreatic organogenesis to contextualize relevant differentiation pathways, then details protocols that guide human pluripotent stem cells through key developmental stages. Methods to enhance cellular maturation and establish higher-performing end products, as well as the therapeutic value of different pancreatic genres, are assessed. Furthermore, crucial gaps are identified, including limited insight into non-beta-endocrine cells, progenitor lineage bias, and off-target differentiation. By chronicling the advancements of all pancreatic organoid classes, the importance of creating more intricate constructs is underscored, which could lead to their broader application.\n                  <\/jats:p>","DOI":"10.1177\/19373341251381368","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T11:56:42Z","timestamp":1758887802000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Stem Cell-Derived Organoids of the Pancreas: Evaluation of Endocrine and Exocrine Modeling Platforms"],"prefix":"10.1177","author":[{"given":"Samantha","family":"Kruzshak","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel S.","family":"Tzanakakis","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering, Tufts University, Medford, Massachusetts, USA."},{"name":"Department of Developmental, Molecular and Cell Biology, Tufts University School of Medicine, Boston, Massachusetts, USA."},{"name":"Graduate Program in Pharmacology and Experimental Therapeutics and Pharmacology Drug Development, Tufts University School of Medicine, Boston, Massachusetts, USA."},{"name":"Clinical and Translational Institute, Tufts Medical Center, Boston, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2020.12.019"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-024-07193-3"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-023-10655-6"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01171-8"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201902042"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2021.04.005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.040"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2021.03.005"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/2050640620905183"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312423"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3201"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14686"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-101512-122405"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2022.909648"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2018.02.009"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-90-481-9060-7_1"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24044027"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.66670"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-021-00831-0"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510790103"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-020-05196-3"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11517-x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-018-4772-2"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","unstructured":"Pancreatic Polypeptide. 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