{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T21:41:41Z","timestamp":1784929301114,"version":"3.55.0"},"reference-count":47,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["GNT1181415"],"award-info":[{"award-number":["GNT1181415"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Future Vision Foundation","award":["Future Vision Foundation Funding Grant FVFG1-011"],"award-info":[{"award-number":["Future Vision Foundation Funding Grant FVFG1-011"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part A"],"abstract":"<jats:p>\n                    Bioengineered corneal constructs are a promising solution to the global shortage of donor tissue. However, most current models lack anatomical curvature and appropriate extracellular matrix (ECM) composition of the native cornea, limiting their relevance for studying graft integration and stromal\u2013endothelial interactions. In this study, we developed a bioprinted, dual-layer corneal model comprising corneal stromal cells laden in type I collagen (Col-I) and a monolayer of corneal endothelial cells supported by collagen type IV (Col-IV). The construct was printed onto a curved support to replicate the posterior curvature of the native cornea. The use of ECM-specific, human-derived collagen bioinks supported high cell viability (&gt;90%) and the formation of a continuous endothelial layer. Histological and immunofluorescence analyses confirmed distinct layering and appropriate cellular morphology and phenotypic marker expression for both corneal stromal and endothelial cells. The construct retained its curvature, transparency, and interfacial integrity over 3 weeks in culture and demonstrated adherence when positioned over an\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    corneal tissue. This anatomically curved, multilayered\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    model offers a physiologically relevant platform for exploring stromal\u2013endothelial architecture and cell interaction in corneal tissue engineering applications.\n                  <\/jats:p>","DOI":"10.1177\/19373341261424272","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T07:13:48Z","timestamp":1772090028000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["A New Bioprinted Dual-Layered Corneal Structure Using Collagen-Based Bioinks"],"prefix":"10.1177","author":[{"given":"Huasheng","family":"Huang","sequence":"first","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, Australia."},{"name":"Save Sight Institute, University of Sydney, Sydney, NSW, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunong","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, Australia."},{"name":"Save Sight Institute, University of Sydney, Sydney, NSW, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Hodge","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, Australia."},{"name":"Save Sight Institute, University of Sydney, Sydney, NSW, Australia."},{"name":"Vision Eye Institute, Chatswood, NSW, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantinos","family":"Pestglou","sequence":"additional","affiliation":[{"name":"Save Sight Institute, University of Sydney, Sydney, NSW, Australia."},{"name":"NSW Tissue Bank, Sydney, NSW, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon G.","family":"Wallace","sequence":"additional","affiliation":[{"name":"Intelligent Polymer Research Institute, Innovation Campus, University of Wollongong, North Wollongong, NSW, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Sutton","sequence":"additional","affiliation":[{"name":"Save Sight Institute, University of Sydney, Sydney, NSW, Australia."},{"name":"Vision Eye Institute, Chatswood, NSW, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"You","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, Australia."},{"name":"Save Sight Institute, University of Sydney, Sydney, NSW, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s2214-109x(17)30393-5"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2015.4776"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/tp.0000000000002938"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/aa92d2"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2018.05.010"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.36702"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-020-02537-6"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.34628"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/polym17091287"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c01509"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/term.2633"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering7030071"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.04.034"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2022.01.034"},{"key":"e_1_3_4_16_2","volume-title":"Corneal Graft Rejection","author":"Gurnani B","year":"2025","unstructured":"Gurnani B, , Czyz CN, , Mahabadi N, et al. 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