{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:37:28Z","timestamp":1777876648538,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2026,3,1]]},"abstract":"<jats:p>\n                    Biological research groups may face a high barrier to entry when constructing custom 3D cell culture devices to investigate multi-tissue interactions\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . Standard fabrication methods such as lithography, etching, or molding are expensive and require specialized equipment and expertise. To address this, we developed an accessible approach for producing polyethylene glycol (PEG)-based cell culture devices using stereolithography 3D printing with a polydimethylsiloxane intermediate mold. Both the intermediate molding steps and the sterilized final device show low cytotoxicity, and the final device swells to predictable dimensions and retains its shape for at least 10 days. We used this approach to develop a human pluripotent stem cell-derived neural spheroid outgrowth model that supports directed neurite extension over 14 days. Together, this method provides a highly customizable, affordable platform for rapid fabrication of PEG-based microphysiological systems for diverse tissue engineering applications.\n                  <\/jats:p>","DOI":"10.1177\/19373384261427525","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T18:53:12Z","timestamp":1773255192000},"page":"80-89","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Low-Cost, Rapid Fabrication of Customizable Polyethylene Glycol-Based Cell Culture Devices"],"prefix":"10.1177","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8555-625X","authenticated-orcid":false,"given":"Emily L.","family":"Pallack","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxwell W.","family":"Oulundsen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Kolpakov","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah R.","family":"Goldberg","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aneth J.","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noah D.","family":"Teaney","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faith E.Y.","family":"Moran","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9597-0936","authenticated-orcid":false,"given":"Nisha R.","family":"Iyer","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj2899"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1188302"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/b411885e"},{"key":"e_1_3_4_5_1","first-page":"71","volume-title":"Methods in Cell Biology [Internet]","author":"Courte J","year":"2018","unstructured":"4.Courte J, , Renault R, , Jan A, , Viovy JL, , Peyrin JM, , Villard C. Reconstruction of directed neuronal networks in a microfluidic device with asymmetric microchannels. In: Methods in Cell Biology [Internet]. Elsevier; 2018. pp. 71\u201395. Available from: https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0091679X1830089X [June 11, 2025]."},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6LC00638H"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2013.0676"},{"key":"e_1_3_4_8_1","unstructured":"7.Purchase PDMS Chips or Fabricate Yourself? [Internet]. uFluidix. 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Combining SLA 3D printing and soft lithography for fast, versatile, and accessible high-resolution fabrication of customised multiscale cell culture devices with complex designs [Internet]. bioRxiv 2022 p. 2022.02.22.481424. https:\/\/www.biorxiv.org\/content\/10.1101\/2022.02.22.481424v1 [May 14, 2024]","journal-title":"bioRxiv"},{"key":"e_1_3_4_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-57816-y"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1LC00744K"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.3c00444"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.11.062"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc20982a"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac071903e"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2019.07.003"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/b404842c"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/adt.2015.662"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.1088\/1367-2630\/16\/11\/115005"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmech.2020.589171"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mame.202400143"},{"key":"e_1_3_4_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2022.11.004"},{"key":"e_1_3_4_24_1","doi-asserted-by":"crossref","unstructured":"23.Cookson M Michael.ward Flores EL. iNDI PiggyBac-TO-hNGN2 transfection protocol Version 1. 2022. 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