{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:47:31Z","timestamp":1782470851090,"version":"3.54.5"},"reference-count":26,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Science and Technology Department of Sichuan Province","award":["2022YFS0077"],"award-info":[{"award-number":["2022YFS0077"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      This research aimed to find the optimal sterilization process for ovarian decellularized extracellular matrix (OV-dECM). OV-dECM was prepared. Four different sterilization processes, such as ultraviolet ray, 1 mg\/L CuCl\n                      <jats:sub>2<\/jats:sub>\n                      + 0.5% H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      , 70% ethanol, and 0.1% peracetic acid (PAA), were evaluated. The sterilization efficiency and the\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in\u00a0vivo<\/jats:italic>\n                      biocompatibility were assessed. Hematoxylin and eosin, 4\u2032,6-diamidino-2-phenylindole staining, and DNA quantitation indicated the successful production of OV-dECM. 0.1% PAA and 70% ethanol achieved better sterilization. The sterilized OV-dECM in the 70% ethanol and 0.1% PAA groups could integrate with human umbilical cord mesenchymal stem cell (between them: 7 days:\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.187; 10 days:\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.293; both\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &gt; 0.05) and keep the cell viability (CCK-8, cell counting kit-8) and cell morphology (F-actin staining). The sterilized OV-dECM in both 70% ethanol and 0.1% PAA groups had good CD31 (a neovascularization maker) fusion indexes (between them:\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.288;\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &gt; 0.05), while \u03b1-smooth muscle actin (another neovascularization maker) in the 0.1% PAA group was higher than that in the 70% ethanol group (between them:\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.012;\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.05). The assessment of CD68, CD86, and CD206 (macrophage phenotype makers) demonstrated that the 0.1% PAA group had better chance to promote the M1\u2013M2 transformation of macrophage than 70% ethanol after the implantation of OV-dECM in mice. In conclusion, 0.1% PAA is the optimal sterilization process for OV-dECM with better sterilization capacity and biocompatibility.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Impact Statement<\/jats:title>\n                    <jats:p>This study identifies 0.1% peracetic acid as the optimal sterilization method for ovarian decellularized extracellular matrix (OV-dECM), resolving a critical barrier to artificial ovary (AO) development. It ensures OV-dECM sterility while preserving biocompatibility, promoting macrophage M1-to-M2 polarization to reduce inflammation. This advances AO translational potential, offering hope for fertility preservation in patients with cancer\/leukemia and guiding standardized OV-dECM sterilization in regenerative medicine.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19373384261448318","type":"journal-article","created":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T08:30:41Z","timestamp":1778315441000},"page":"215-229","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["0.1% Peracetic Acid\u00a0Treatment Is the Optimal Process for Ovarian Decellularized Extracellular Matrix"],"prefix":"10.1177","volume":"32","author":[{"given":"MeyLign","family":"Long","sequence":"first","affiliation":[{"name":"Reproductive Medical Department of West China 2nd University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Touch","sequence":"additional","affiliation":[{"name":"Reproductive Medical Department of West China 2nd University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Yang","sequence":"additional","affiliation":[{"name":"Reproductive Medical Department of West China 2nd University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Medical Department of West China 2nd University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4638-1713","authenticated-orcid":false,"given":"Zhun","family":"Xiao","sequence":"additional","affiliation":[{"name":"Reproductive Medical Department of West China 2nd University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,5,9]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/jfb13040165"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(21)00006-1"},{"key":"e_1_3_2_4_2","first-page":"e84657","article-title":"Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression","volume":"12","author":"Pierson Smela MD","year":"2023","unstructured":"Pierson Smela MD, , Kramme CC, , Fortuna PRJ, et al. Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression. 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Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal. 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