{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T02:17:12Z","timestamp":1785205032392,"version":"3.55.0"},"reference-count":53,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["P20GM103427"],"award-info":[{"award-number":["P20GM103427"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"crossref","award":["R01 HL155439"],"award-info":[{"award-number":["R01 HL155439"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["DK106829"],"award-info":[{"award-number":["DK106829"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tissue Engineering Part C: Methods"],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:p>\n                    Hematopoietic stem and progenitor cells (HSPCs) are precisely organized within specialized bone marrow niches containing hematopoietic and nonhematopoietic cell types. Physiologically accurate human models of this niche environment that include functional HSPCs have been lacking until recently. Using induced pluripotent stem cells (iPSCs) grown in mixed-matrix hydrogels, vascularized three-dimensional human bone marrow organoids (hBMOs) can now be generated that contain mesenchymal, endothelial, and hematopoietic cells, all vital components of the bone marrow niche. While hBMOs have been shown to support engraftment of normal and malignant cells, there are numerous opportunities to use hBMOs for developing a wider experimental toolkit to test gene functions and stress responses. Here, we establish two new applications for the hBMO platform. First, we demonstrate successful engraftment of gene-edited CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells from healthy donors, enabling direct investigation of gene-specific effects on human hematopoiesis in a defined microenvironment. Gene-edited engrafted HSPCs are maintained in hBMOs for 7 days and undergo multilineage differentiation. Second, we adapted a method to induce stress erythropoiesis\u2014a response to acute anemia in mice and humans\u2014in hBMOs, resulting in robust expansion of immunophenotypically defined hematopoietic progenitors and erythroid populations. These two advances expand the laboratory uses for hBMOs and establish this system as a versatile platform for studying human hematopoiesis and erythropoiesis, stress responses, and gene functions within a physiologically relevant bone marrow microenvironment.\n                  <\/jats:p>","DOI":"10.1177\/19373384261463760","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T11:15:06Z","timestamp":1783682106000},"page":"267-276","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Applications of the Human Bone Marrow Organoid System to Study Hematopoiesis and Anemia Stress"],"prefix":"10.1177","volume":"32","author":[{"given":"Meg A.","family":"Schaefer","sequence":"first","affiliation":[{"name":"Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian R.","family":"Black","sequence":"additional","affiliation":[{"name":"Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Katherine","family":"Hyde","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6832-3947","authenticated-orcid":false,"given":"Daniel A.","family":"Kuppers","sequence":"additional","affiliation":[{"name":"Human Biology Division, Fred Hutchinson Cancer Center, Seattle, Washington, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1946-625X","authenticated-orcid":false,"given":"Kyle J.","family":"Hewitt","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0439-6"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1104-8"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.53"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015499"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.04.054"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.03.024"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2013.05.001"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11926"},{"key":"e_1_3_4_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.09.014"},{"key":"e_1_3_4_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.08.017"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.10.016"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.09.053"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.10.020"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2021.10.006"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2021.06.006"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2010.156"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2020.113794"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2019.233320"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-02-0703"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI41291"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-07-591453"},{"key":"e_1_3_4_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.4.1451"},{"issue":"5","key":"e_1_3_4_24_1","first-page":"737","article-title":"\u2018Fetal\u2019 erythropoiesis following bone marrow transplantation as estimated by the number of F cells in the peripheral blood","volume":"14","author":"Meletis J","year":"1994","unstructured":"23.Meletis J, , Papavasiliou S, , Yataganas X, et al. \u2018Fetal\u2019 erythropoiesis following bone marrow transplantation as estimated by the number of F cells in the peripheral blood. 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