{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:07:53Z","timestamp":1785769673958,"version":"3.56.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Ministry of Higher Education & the National Center for Scientific and Technical Research (CNRST) of Morocco","award":["Cov\/2020\/23 \u2014 T1"],"award-info":[{"award-number":["Cov\/2020\/23 \u2014 T1"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Genetic Testing and Molecular Biomarkers"],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The COVID-19 was the most amazing pandemic of the early third millennium, with significant variability in infection rates across regions worldwide. Genetic factors, particularly the ACE2 gene, coding for a common receptor implicated in facilitating SARS-CoV-2 entry into human cells, could play a crucial role in susceptibility to infection. Our study aimed to analyze the allelic frequency of four SNPs in ACE2 gene (rs4646127, rs2074192, rs2285666, rs112171234) and evaluate their correlations with COVID-19 infection rates in four regional samples from Morocco.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      The total sample (\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 200) was obtained from: Rif (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 50), Doukkala (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 50), Ouarzazate (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 49), and, Guelmim (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 51). Genomic DNA was extracted using the GeneJET\u2122 kit and genotyped by real-time PCR using TaqMan technology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Analyses revealed significant spatial variations in the frequencies of alternative alleles of the studied SNPs, ranging from 0% to 74%. A significant positive correlation was observed between the frequency of alternative G allele of the rs4646127 and the infection rate variation (\n                      <jats:italic toggle=\"yes\">r<\/jats:italic>\n                      = 0.96;\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.03;\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 4). Furthermore, significant differences reached 54.17% with worldwide populations (from North Africa, sub-Saharan Africa, Europe, Latino-America, East Asia, and South Asia) for the total SNP\u2019s comparisons.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Our results suggest a significant positive correlation between the frequency of the alternative allele of the ACE2 gene polymorphism rs4646127 and COVID-19 regional infection rates in Morocco. This property could be exploited to consider this SNP as a potential biomarker for identifying at-risk groups. However, due to the limited number of regional samples, our results for all the analyzed SNPs remain exploratory and would require confirmation through larger-scale studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19450265261468935","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T11:23:12Z","timestamp":1783682592000},"page":"208-217","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Diversity of the ACE2 Gene and Susceptibility to COVID-19 in Morocco Population"],"prefix":"10.1177","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7291-0247","authenticated-orcid":false,"given":"Nourdin","family":"Harich","sequence":"first","affiliation":[{"name":"Laboratory of Anthropogenetics, Biotechnology and Health, Department of Biology, Faculty of Sciences, Choua\u00efb Doukkali University, El Jadida, Morocco."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3764-2835","authenticated-orcid":false,"given":"Hamid","family":"Farhane","sequence":"additional","affiliation":[{"name":"Laboratory of Anthropogenetics, Biotechnology and Health, Department of Biology, Faculty of Sciences, Choua\u00efb Doukkali University, El Jadida, Morocco."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soufiane","family":"Karchali","sequence":"additional","affiliation":[{"name":"Laboratory of Anthropogenetics, Biotechnology and Health, Department of Biology, Faculty of Sciences, Choua\u00efb Doukkali University, El Jadida, Morocco."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majida","family":"Motrane","sequence":"additional","affiliation":[{"name":"Laboratory of Anthropogenetics, Biotechnology and Health, Department of Biology, Faculty of Sciences, Choua\u00efb Doukkali University, El Jadida, Morocco."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima-Ezzahra","family":"Anaibar","sequence":"additional","affiliation":[{"name":"Laboratory of Anthropogenetics, Biotechnology and Health, Department of Biology, Faculty of Sciences, Choua\u00efb Doukkali University, El Jadida, Morocco."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Said Nassor","family":"Abeid","sequence":"additional","affiliation":[{"name":"Laboratory of Anthropogenetics, Biotechnology and Health, Department of Biology, Faculty of Sciences, Choua\u00efb Doukkali University, El Jadida, Morocco."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Abidi","sequence":"additional","affiliation":[{"name":"Higher Institute of Nursing and Health Techniques of Casablanca, Ministry of Health and Social Protection, Casablanca, Morocco."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderraouf","family":"Hilali","sequence":"additional","affiliation":[{"name":"Faculty of Sciences and Techniques, Hassan First University, Settat, Morocco."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.579415"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-022-01782-6"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40246-020-00290-4"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/FEBS.15375"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-78170-3"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/S10096-020-04138-6"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/PATH.5471"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-020-0147-1"},{"issue":"19","key":"e_1_3_3_10_1","first-page":"2654","article-title":"The influence of angiotensin-converting enzyme 2 gene polymorphisms on type 2 diabetes mellitus and coronary heart disease","volume":"17","author":"Chaoxin J","year":"2013","unstructured":"Chaoxin J, , Daili S, , Yanxin H, et al. 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