{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:36:01Z","timestamp":1792787761010,"version":"4.5.0"},"reference-count":49,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"NASI Senior Scientist Platinum Jubilee Fellowship"},{"name":"Indian Council of Medical Research","award":["5\/13\/25\/2019\/NCD-III"],"award-info":[{"award-number":["5\/13\/25\/2019\/NCD-III"]}],"id":[{"id":"https:\/\/ror.org\/0492wrx28","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Council of Scientific and Industrial Research, India","award":["09\/030(0083)\/2019-EMR-I"],"award-info":[{"award-number":["09\/030(0083)\/2019-EMR-I"]}],"id":[{"id":"https:\/\/ror.org\/021wm7p51","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Centre International de Recherche sur le Cancer","award":["CRA\/ETR\/2019\/1"],"award-info":[{"award-number":["CRA\/ETR\/2019\/1"]}],"id":[{"id":"https:\/\/ror.org\/00v452281","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Genetic Testing and Molecular Biomarkers"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>This study aimed to evaluate the triage performance of microRNA (miRNA) biomarkers on self-collected, high-risk human papillomavirus (HR-HPV)-positive cervical swabs to discern cervical intraepithelial neoplasia II\u2013III or high-grade squamous intraepithelial lesions (HSIL) in Indian women for precise diagnosis and treatment prioritization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Through\n                      <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                      analysis, miR-135b-5p and miR-21-5p were identified as upregulated and amplified miRNAs in cervical cancer (CaCx). Quantitative real-time PCR was used to assess miR-135b-5p and miR-21-5p and their targeting, LIM domain-containing protein 1 (LIMD1) and von Hippel\u2013Lindau (VHL) messenger RNA (mRNA) expression, in a discovery cohort (\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 42; normal cervical epithelium [\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 9], CIN [\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 12], and CaCx [\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 21] tissue samples). Thereafter, miRNA expression was validated in an independent set of self-collected cervical swabs (\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 243; HR-HPV-positive [asymptomatic (\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 52), low-grade squamous intraepithelial lesions (LSIL) (\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 50), and HSIL (\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 52), CaCx (\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 35)] and HPV-negative normal [\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 54]). Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic performance of these two candidate miRNAs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      In discovery-phase samples, miR-135b-5p showed progressive upregulation from CIN (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.056) to CaCx (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.012), while miR-21-5p showed significant overexpression at CIN (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.011) and CaCx (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.005) compared to normal tissue. Concurrently, significant downregulation of LIMD1\/VHL mRNA expression was observed in the same CaCx samples. In the validation cohort, miR-135b-5p and miR-21-5p were significantly overexpressed in HSIL samples compared to lower-grade lesions (\u2264LSIL), with a corresponding downexpression of LIMD1\/VHL. ROC curve analysis demonstrated high sensitivity and specificity for miR-21-5p and miR-135b-5p\u2014both individually and in combination\u2014for distinguishing HR-HPV + HSIL from LSIL, asymptomatic, or HPV-negative normal samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>miR-135b-5p and miR-21-5p constitute a feasible triage biomarker on noninvasive, self-collected cervical swab samples for early, precise diagnosis of women with HR-HPV-positive HSIL, requiring treatment prioritization in low-resource settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19450265261491098","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:49:16Z","timestamp":1790268556000},"page":"264-275","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical Evaluation of miR-135b-5p and miR-21-5p Expression on Self-Collected Cervical Swab Samples for Triaging HR-HPV-Positive High-Grade Preneoplastic Lesions in India"],"prefix":"10.1177","volume":"30","author":[{"given":"Farhin","family":"Sultana","sequence":"first","affiliation":[{"name":"Department of Oncogene Regulation, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananya","family":"De","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Zurich, Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreeya","family":"Bose","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anirban","family":"Roychowdhury","sequence":"additional","affiliation":[{"name":"Department of Oncogene Regulation, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masmuda","family":"Khatun","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puja","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Vernekar","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipanwita","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayanta","family":"Chakrabarti","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranajit Kumar","family":"Mandal","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinmay Kumar","family":"Panda","sequence":"additional","affiliation":[{"name":"Department of Oncogene Regulation, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8144-4345","authenticated-orcid":false,"given":"Sankhadeep","family":"Dutta","sequence":"additional","affiliation":[{"name":"Department of Oncogene Regulation, Chittaranjan National Cancer Institute, Kolkata, West Bengal, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_3_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-89388-w"},{"key":"e_1_3_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm15031162"},{"key":"e_1_3_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.06.095"},{"issue":"4","key":"e_1_3_7_5_1","first-page":"9865","article-title":"Down-regulation of microRNA-432 in cervical mucus as a pioneer marker in early detection of cervical cancer","volume":"25","author":"Asuffix AMAR","year":"2021","unstructured":"Asuffix AMAR. 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