{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:26:54Z","timestamp":1777386414730,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2018,11,20]],"date-time":"2018-11-20T00:00:00Z","timestamp":1542672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["MAT2016-76039-C4-2-R (CC)"],"award-info":[{"award-number":["MAT2016-76039-C4-2-R (CC)"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["PI16\/01315 (MM)"],"award-info":[{"award-number":["PI16\/01315 (MM)"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Rhinol\ufffdAllergy"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The respiratory epithelium is frequently infected by the respiratory syncytial virus, resulting in inflammation, a reduction in cilia activity and an increase in the production of mucus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, an automatic method has been proposed to characterize the ciliary motility from cell cultures by means of a motility index using a dense optical flow algorithm. This method allows us to determine the ciliary beat frequency (CBF) together with a ciliary motility index of the cells in the cultures. The object of this analysis is to automatically distinguish between normal and infected cells in a culture.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The method was applied in 2 stages. It was concluded from the first stage that the CBF is not a good enough indicator to discriminate between the control and infected cultures. However, the ciliary motility index does succeed in discriminating between the control and infected cultures using the t test with a value t\u2009=\u20096.46 and P\u2009&lt;\u2009.001. In the second stage, it has been shown that the ciliary motility index did not differ significantly between patients, and the analysis of variance test gives \u03b1\u2009=\u20090.05, F\u2009=\u20091.61, P\u2009=\u2009.20. A threshold for this index has been determined using a receiver operating characteristics analysis that gives an area under the curve of 0.93.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We have obtained a ciliary motility index that is able to discriminate between control and infected cultures after the eighth postinfection day. After infection, there is a rapid cilia loss of the cells and the measured CBF corresponds to the remaining noninfected cells. This is why the CBF does not discriminate between the control and the infected cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1945892418811324","type":"journal-article","created":{"date-parts":[[2018,11,20]],"date-time":"2018-11-20T07:08:46Z","timestamp":1542697726000},"page":"121-128","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["A Ciliary Motility Index for Activity Measurement in Cell Cultures With Respiratory Syncytial Virus"],"prefix":"10.1177","volume":"33","author":[{"given":"Eduardo","family":"Parrilla","sequence":"first","affiliation":[{"name":"Instituto de Biomec\u00e1nica de Valencia, Universitat Polit\u00e8cnica de Val\u00e8ncia, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Armengot","sequence":"additional","affiliation":[{"name":"Departament de Cirurgia, Universitat de Val\u00e8ncia, Hospital Universitari i Polit\u00e8cnic la Fe, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Mata","sequence":"additional","affiliation":[{"name":"Departament de Patologia, Universitat de Val\u00e8ncia, Valencia, Spain"},{"name":"Instituto de investigaci\u00f3n Sanitaria, Valencia, Spain"},{"name":"Centro de Enfermedades en Red de Enfermedades Respiratorias, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Carda","sequence":"additional","affiliation":[{"name":"Departament de Patologia, Universitat de Val\u00e8ncia, Valencia, Spain"},{"name":"Instituto de investigaci\u00f3n Sanitaria, Valencia, Spain"},{"name":"Centro de Enfermedades en Red de Bioingenier\u00eda, Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juilo","family":"Cortijo","sequence":"additional","affiliation":[{"name":"Centro de Enfermedades en Red de Enfermedades Respiratorias, Madrid, Spain"},{"name":"Departament de Farmacologia, Universitat de Val\u00e8ncia, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2825-3646","authenticated-orcid":false,"given":"David","family":"Moratal","sequence":"additional","affiliation":[{"name":"Centro de Biomateriales e Ingenier\u00eda Tisular, Universitat Polit\u00e8cnica de Val\u00e8ncia, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dami\u00e1n","family":"Ginestar","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Matem\u00e1tica Multidisciplinar, Universitat Polit\u00e8cnica de Val\u00e8ncia, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 L.","family":"Hueso","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Matem\u00e1tica Multidisciplinar, Universitat Polit\u00e8cnica de Val\u00e8ncia, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4183-7195","authenticated-orcid":false,"given":"Jaime","family":"Riera","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Matem\u00e1tica Multidisciplinar, Universitat Polit\u00e8cnica de Val\u00e8ncia, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,11,20]]},"reference":[{"key":"bibr1-1945892418811324","unstructured":"Collins PL, Chanock RM, Murphy BR. 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