{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:55:01Z","timestamp":1790801701837,"version":"4.1.0"},"reference-count":30,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2018,12,25]],"date-time":"2018-12-25T00:00:00Z","timestamp":1545696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["5P30DK072482-05"],"award-info":[{"award-number":["5P30DK072482-05"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["1 R01 HL133006-03"],"award-info":[{"award-number":["1 R01 HL133006-03"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010485","name":"American Rhinologic Society","doi-asserted-by":"publisher","award":["Career Development Award 2017"],"award-info":[{"award-number":["Career Development Award 2017"]}],"id":[{"id":"10.13039\/100010485","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["ROWE15R0"],"award-info":[{"award-number":["ROWE15R0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Rhinol\ufffdAllergy"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Methods to improve the clinical efficacy of currently available antibiotics against multidrug resistant bacteria in cystic fibrosis (CF) chronic rhinosinusitis (CRS) are greatly needed. Ivacaftor, a cystic fibrosis transmembrane conductance regulator potentiator, was recently identified as having potentially beneficial off-target effects as a weak inhibitor of bacterial DNA gyrase and topoisomerase IV. The objective of the current study is to evaluate whether ivacaftor enhances the antimicrobial activity of ciprofloxacin against Pseudomonas aeruginosa.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The planktonic growth of the PAO-1 strain of P. aeruginosa was studied in the presence of ciprofloxacin and\/or ivacaftor. Effects were measured according to optical density of cultured PAO-1 at 600 nm. For a static PAO-1 biofilm assay, the PAO-1 strain was inoculated and cultured for 72 h in the presence of the drugs. Formed PAO-1 biofilms were quantified by crystal violet staining and imaged with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PAO-1 growth was significantly reduced in the presence of ivacaftor (8 or 16 \u00b5g\/mL) and ciprofloxacin (0.02 or 0.05 \u00b5g\/mL) compared to ciprofloxacin alone ( P\u2009&lt;\u2009.001). Similarly, ivacaftor (8 or 16 \u00b5g\/mL) showed a significant reduction of PAO-1 biofilms when treated with 0.05 \u00b5g\/mL of ciprofloxacin. Significant synergism was noted between ciprofloxacin and 16\u2009\u00b5g\/mL of ivacaftor ( P\u2009&lt;\u2009.0001) in reducing planktonic growth and biofilm formation. Quantitative measurements with crystal violet staining were correlated to CLSM and SEM images.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Ivacaftor enhanced ciprofloxacin\u2019s antimicrobial activity against P. aeruginosa. Further studies evaluating the efficacy of ivacaftor\/ciprofloxacin combination for P. aeruginosa for CF CRS are warranted.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1945892418815615","type":"journal-article","created":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T00:49:31Z","timestamp":1545785371000},"page":"129-136","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":48,"title":["Ivacaftor, a Cystic Fibrosis Transmembrane Conductance Regulator Potentiator, Enhances Ciprofloxacin Activity Against\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1177","volume":"33","author":[{"given":"Do-Yeon","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama"},{"name":"Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Jin","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calvin","family":"Mackey","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Skinner","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"McCormick","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradford A.","family":"Woodworth","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama"},{"name":"Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,12,25]]},"reference":[{"key":"bibr1-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199608000-00018"},{"key":"bibr2-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1378\/chest.121.1.40"},{"key":"bibr3-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-015-0591-4"},{"key":"bibr4-1945892418815615","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2008.22.3178"},{"key":"bibr5-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/44.1.43"},{"key":"bibr6-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1097\/01.moo.0000124936.46948.6a"},{"key":"bibr7-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1016\/S1569-1993(13)00150-1"},{"key":"bibr8-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22067"},{"key":"bibr9-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2011.58"},{"key":"bibr10-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2013.09.004"},{"key":"bibr11-1945892418815615","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2017.31.4461"},{"key":"bibr12-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.6b00035"},{"key":"bibr13-1945892418815615","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2013.27.3919"},{"key":"bibr14-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0000000000000107"},{"key":"bibr15-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066955"},{"key":"bibr16-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081589"},{"key":"bibr17-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2014.02.004"},{"key":"bibr18-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124124"},{"key":"bibr19-1945892418815615","doi-asserted-by":"publisher","DOI":"10.1038\/srep16043"},{"key":"bibr20-1945892418815615","doi-asserted-by":"crossref","unstructured":"Merritt JH, Kadouri DE, O\u2019Toole GA. 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