{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T07:31:36Z","timestamp":1783063896499,"version":"3.54.6"},"reference-count":54,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T00:00:00Z","timestamp":1562112000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"the program for Changjiang Scholars and Innovative Research Team","award":["IRT13082"],"award-info":[{"award-number":["IRT13082"]}]},{"name":"the National Key R&D Program of China","award":["2016YFC20160905200"],"award-info":[{"award-number":["2016YFC20160905200"]}]},{"name":"the Capital Health Development Foundation","award":["2016-1-2052"],"award-info":[{"award-number":["2016-1-2052"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81420108009, 81400444, 81470678 and 81630023"],"award-info":[{"award-number":["81420108009, 81400444, 81470678 and 81630023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Scientific Research and Training Program of Beijing Medical Administration","award":["PX2019007"],"award-info":[{"award-number":["PX2019007"]}]},{"name":"the Priming Scientific Research Foundation for the Senior Researcher in Beijing TongRen Hospital, Capital Medical University","award":["2017-YJJ-GGL-005"],"award-info":[{"award-number":["2017-YJJ-GGL-005"]}]},{"name":"Beijing Scientific and Technological Overall Plan","award":["Z171100000117002"],"award-info":[{"award-number":["Z171100000117002"]}]},{"name":"the Natural Science Foundation of Beijing","award":["7194247"],"award-info":[{"award-number":["7194247"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Rhinol\ufffdAllergy"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP) is a distinct phenotype of chronic rhinosinusitis with nasal polyps (CRSwNP), with many significantly different clinical features from non-eosinophilic chronic rhinosinusitis with nasal polyps (non-ECRSwNP). Thus, identification of subtypes is crucial for precise treatment. Immunohistology is a reliable way to present the subtypes; however, the results mainly depend on the observation of pathologist, and the method with automatic readout and the corresponding biomarkers is lacking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The purpose of our research was to explore the predictive value of quantitative reverse transcription polymerase chain reaction (qRT-PCR) as an alternative method and messenger RNA (mRNA) expression of Charcot\u2013Leyden crystals (CLC) as a corresponding target for ECRSwNP, which may benefit the automatized judgment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>CLC mRNA levels in tissue samples from 48 CRSwNP patients and 10 controls were evaluated by quantitative real-time PCR. Hematoxylin and eosin staining was performed for histological assessment of CRSwNP and subtyping as ECRSwNP and non-ECRSwNP. Factors associated with ECRSwNP were determined with logistic regression analysis, the predictive value was presented by a receiver operating characteristic (ROC) curve, and optimal cutoff points of the predictors were identified as the Youden index.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>mRNA level of CLC in ECRSwNP was significantly elevated compared to either non-ECRSwNP group or control group, with no significant difference between non-ECRSwNP patients and controls. CLC mRNA levels were positively correlated with percentages of tissue eosinophil and peripheral blood eosinophil ( P\u2009&lt;\u2009.001, r\u2009=\u2009.683; P\u2009=\u2009.003, r\u2009=\u2009.420, respectively). Logistic regression analysis revealed CLC mRNA level and blood eosinophil percentages were prediagnosis factors ( P\u2009=\u2009.007, P\u2009=\u2009.045, respectively) for ECRSwNP. ROC curves analysis indicated the area under the curve (AUC) of CLC mRNA level was 0.948 which was superior to the blood eosinophil percentage (AUC\u2009=\u20090.797; P\u2009=\u2009.044) as an optimal biomarker to predict ECRSwNP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CLC mRNA levels based on the qRT-PCR may serve as a reliable and alternative method for the identification of ECRSwNP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1945892419860646","type":"journal-article","created":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T22:46:48Z","timestamp":1562194008000},"page":"671-680","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Predictive Significance of Charcot\u2013Leyden Crystals for Eosinophilic Chronic Rhinosinusitis With Nasal Polyps"],"prefix":"10.1177","volume":"33","author":[{"given":"Chang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, P.R. 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