{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:16:03Z","timestamp":1788405363176,"version":"build-2803163510"},"reference-count":30,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T00:00:00Z","timestamp":1566777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\/CIRG\/1458\/2016"],"award-info":[{"award-number":["NMRC\/CIRG\/1458\/2016"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"The National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["1670909"],"award-info":[{"award-number":["1670909"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"The National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81873690"],"award-info":[{"award-number":["81873690"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001349","name":"National Medical Research Council of Singapore","doi-asserted-by":"crossref","award":["OFYIRG17nov065"],"award-info":[{"award-number":["OFYIRG17nov065"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme 2017"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Rhinol\ufffdAllergy"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gene expression patterns (particularly, cilia-associated genes) of nasal mucosa, the first-line defense system, in allergic rhinitis (AR) are not well understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We sought to screen for AR-associated genes in inferior turbinate (IT) from patients with AR, and to validate the expression of common cilia-related genes and ciliary shedding.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Prime View\u2122 Human Gene Expression Array, which consisted of more than 530\u00a0000 probes covering more than 36\u00a0000 transcripts and variants, was employed to compare individual gene expression of ITs from control subjects (n\u2009=\u200911) and patients with AR (n\u2009=\u200919). Gene ontology (GO) analysis was performed with Cytoscape software. Eight of the common cilia-related genes were validated with quantitative polymerase chain reaction. We applied a semiquantitative scoring system for immunofluorescence assay to demonstrate ciliary shedding in 5 areas per paraffin section, with individual sections being scored between 0 (normal ciliary distribution) and 1 (ciliary shedding).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared with control subjects, 160 (38 upregulated and 122 downregulated) genes were differentially expressed for at least 2 folds (all P\u2009&lt;\u2009.05) in AR. Seven GO categories were significantly enriched, 4 of which were related to cilium assembly and motility. Quantitative polymerase chain reaction validated the predicted direction of change for common cilia-related gene expression. The ciliary distribution score was significantly higher (more prominent ciliary shedding) in AR than in controls ( P\u2009&lt;\u2009.05).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The significant aberrant cilia-related gene expression, revealed by microarray assays, might be the critical driver of AR where ciliary shedding is prominent.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1945892419871795","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:48:01Z","timestamp":1566866881000},"page":"50-58","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Microarray Assay Reveals Ciliary Abnormalities of the Allergic Nasal Mucosa"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2113-5858","authenticated-orcid":false,"given":"Yang","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou, China"},{"name":"State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, Guangzhou Institute for Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China"},{"name":"Department of Otolaryngology, National University of Singapore, National University Health System, Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-jie","family":"Guan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, Guangzhou Institute for Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China"},{"name":"Department of Otolaryngology, National University of Singapore, National University Health System, Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-chao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Sen","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, National University of Singapore, National University Health System, Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head & Neck Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-yu","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, The 5th Affiliated Hospital of Sun Yat-Sen University, Zhuhai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-xue","family":"Zi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, National University of Singapore, National University Health System, Singapore, Singapore"},{"name":"Department of Otolaryngology, The Second Hospital of Shandong University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand Kumar","family":"Andiappan","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, The Second Hospital of Shandong University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin-tai","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, National University of Singapore, National University Health System, Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian-hui","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou, 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