{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:30:09Z","timestamp":1777386609687,"version":"3.51.4"},"reference-count":27,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T00:00:00Z","timestamp":1592524800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology, Japan","doi-asserted-by":"crossref","award":["16K15721"],"award-info":[{"award-number":["16K15721"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology, Japan","doi-asserted-by":"crossref","award":["17K16920"],"award-info":[{"award-number":["17K16920"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"apan Agency for Medical Research and Development","award":["JP16ek0109062."],"award-info":[{"award-number":["JP16ek0109062."]}]},{"name":"apan Agency for Medical Research and Development","award":["JP17ek0410040"],"award-info":[{"award-number":["JP17ek0410040"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Rhinol\ufffdAllergy"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Hyaluronan is one of the major extracellular matrixes in chronic rhinosinusitis (CRS) associated with tissue remodeling. Prostaglandin D\n                      <jats:sub>2<\/jats:sub>\n                      (PGD\n                      <jats:sub>2<\/jats:sub>\n                      ) is also associated with the pathogenesis of CRS. However, little is known about whether PGD\n                      <jats:sub>2<\/jats:sub>\n                      regulates hyaluronan production by human airway fibroblasts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      We sought to determine the effect of PGD\n                      <jats:sub>2<\/jats:sub>\n                      on the mRNA expression of three isoforms of membrane-bound hyaluronic acid synthase (HAS1, HAS2 and HAS3) in fibroblasts, the major source of hyaluronan production, derived from CRS patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Nasal polyp-derived fibroblasts (NPDF) and uncinate tissue-derived fibroblasts (UTDF) were established from CRS patients with nasal polyps and those without, respectively. These fibroblasts were stimulated with PGD\n                      <jats:sub>2<\/jats:sub>\n                      or PGD\n                      <jats:sub>2<\/jats:sub>\n                      receptor (DP\/CRTH2)-selective agonists in the presence or absence of receptor-selective antagonists. mRNA levels for HAS1, HAS2 and HAS3 were determined by real-time quantitative PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      PGD\n                      <jats:sub>2<\/jats:sub>\n                      (1\u2009\u00b5M) significantly enhanced HAS1 but not HAS2 or HAS3 mRNA expression by NPDF. Enhanced HAS1 mRNA expression was also obtained by stimulation with a DP receptor-selective agonist, but not with a CRTH2 receptor-selective agonist. In addition, PGD\n                      <jats:sub>2<\/jats:sub>\n                      -induced HAS1 mRNA expression was significantly inhibited by pre-treatment with DP receptor-selective antagonists. Similar induction of PGD\n                      <jats:sub>2<\/jats:sub>\n                      -induced HAS1 mRNA expression was seen in UTDF.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      PGD\n                      <jats:sub>2<\/jats:sub>\n                      selectively stimulates HAS1 mRNA expression in local fibroblasts in CRS via DP, but not CRTH2, receptors.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1945892420932781","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T07:15:56Z","timestamp":1592550956000},"page":"44-51","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of Prostaglandin D\n                    <sub>2<\/sub>\n                    on mRNA Expression of Three Isoforms of Hyaluronic Acid Synthase in Nasal Polyp Fibroblasts"],"prefix":"10.1177","volume":"35","author":[{"given":"Yuji","family":"Hirata","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2014Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan"},{"name":"Department of Otorhinolaryngology, Kagawa Prefectural Central Hospital, Takamatsu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7810-5844","authenticated-orcid":false,"given":"Shin","family":"Kariya","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kengo","family":"Kanai","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan"},{"name":"Department of Otorhinolaryngology, Kagawa Prefectural Central Hospital, Takamatsu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tazuko","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sei-ichiro","family":"Makihara","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Kagawa Rosai Hospital, Marugame, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5441-9796","authenticated-orcid":false,"given":"Ryotaro","family":"Omichi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head & Neck Surgery, The University of Iowa, Iowa City, Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takaya","family":"Higaki","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takenori","family":"Haruna","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Himeji St. Mary\u2019s Hospital, Himeji, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiko","family":"Oka","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, International University of Health and Welfare School of Medicine, Narita, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunori","family":"Nishizaki","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head 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