{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:41:03Z","timestamp":1792093263164,"version":"4.4.0"},"reference-count":35,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Rhinol\ufffdAllergy"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Odontogenic sinusitis (ODS) is a common cause of unilateral infectious maxillary sinusitis but is frequently underdiagnosed due to nonspecific symptoms, subtle dental findings on imaging, and limitations of traditional bacterial cultures\u2014particularly for anaerobic organisms. Next-generation DNA sequencing (NGS) may enhance detection of odontogenic bacteria.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study compared bacterial detection between standard cultures versus NGS in patients with confirmed ODS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty consecutive adults with confirmed ODS were enrolled. Maxillary sinus purulence was collected endoscopically and submitted for routine aerobic and anaerobic bacterial cultures and for NGS-based microbial profiling using 16S rRNA gene sequencing. Detected organisms were categorized by aerotolerance and ODS relationship, and likely nasal colonizers were excluded from analyses. Alpha diversity, beta diversity, and compositional differences between methods were analyzed statistically.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      NGS detected significantly more bacterial species per specimen than culture (mean 4.9 vs 2.7;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009=\u2009.0015). Compared to cultures, NGS identified more anaerobic bacteria (4.2 vs 0.9;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009=\u2009.00004) and more ODS-related species (4.4 vs 1.0;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009=\u2009.0001), while detection of aerobic species was similar between methods. Cultures also often yielded nonspeciated results, whereas NGS consistently revealed polymicrobial communities dominated by oral anaerobes. Microbial composition differed significantly between detection methods.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In confirmed ODS, NGS detected significantly more anaerobic and ODS-related species than culture from the same specimens. These findings suggest that culture alone may underestimate odontogenic contributions to purulent sinusitis. Future studies should explore he utility of NGS as an adjunctive tool to facilitate diagnosis and treatment in the setting of possible ODS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19458924261474104","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:40:16Z","timestamp":1785422416000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Microbial Diversity in Odontogenic Sinusitis: Next-Generation Sequencing Versus Culture"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1270-2272","authenticated-orcid":false,"given":"Abdurrahman","family":"Abdurrob","sequence":"first","affiliation":[{"name":"Henry Ford Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do-Yeon","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2014Head and Neck Surgery, University of Alabama, Birmingham, AL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob G.","family":"Eide","sequence":"additional","affiliation":[{"name":"Mayo Clinic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrita","family":"Ray","sequence":"additional","affiliation":[{"name":"Henry Ford Health"},{"name":"Department of Otolaryngology\u2014Head and Neck Surgery, Michigan State University College of Human Medicine, Detroit, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob S.","family":"Ancira","sequence":"additional","affiliation":[{"name":"MicroGenDX, Lubbock, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Tipton","sequence":"additional","affiliation":[{"name":"MicroGenDX, Lubbock, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary","family":"Hanna","sequence":"additional","affiliation":[{"name":"Henry Ford Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Craig","sequence":"additional","affiliation":[{"name":"Henry Ford Health"},{"name":"Department of Otolaryngology\u2014Head and Neck Surgery, Michigan State University College of Human Medicine, Detroit, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22777"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjoto.2021.102925"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22434"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2024.07.001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2014.12.006"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/19458924221114941"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22598"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/lary.32376"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21212"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/1945892420941702"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1177\/1945892418804975"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22627"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/lary.70323"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.031476-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21629"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2017.01.023"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.MLG.0000157332.17291.FC"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5721-5732.2005"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.4103\/jomfp.JOMFP_304_18"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.euros.2023.09.008"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13884"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008511"},{"key":"e_1_3_3_24_2","volume-title":"Medical Microbiology, 4th edition","author":"Davis CP","year":"1996","unstructured":"Davis CP. 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