{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T17:50:42Z","timestamp":1782496242196,"version":"3.54.5"},"reference-count":51,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100015657","name":"University of Tehran Science and Technology Park","doi-asserted-by":"crossref","award":["7108017\/6\/43"],"award-info":[{"award-number":["7108017\/6\/43"]}],"id":[{"id":"10.13039\/501100015657","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"abstract":"<jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>Nanoliposomes (NLs) are a rich source of phospholipids, including phosphatidylcholine, and can encapsulate and release hydrophilic and hydrophobic compounds, separately or simultaneously, to different cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>This study aimed to assess the impact of NLs or nanomicelles (NMs) on characteristics of frozen\u2013thawed bull sperm.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Semen samples were collected with an artificial vagina twice weekly from five bulls. Ejaculates that met minimum characteristics (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 36) were pooled, extended with 1, 2, or 3% NL or NM, and cryopreserved. Frozen\u2013thawed sperm were assessed regarding motility (computer-assisted sperm analysis), morphology, and plasma membrane integrity (eosin-nigrosin), plasma membrane functionality (HOST), acrosome integrity (\n                      <jats:italic toggle=\"yes\">Pisum sativum<\/jats:italic>\n                      agglutinin), and fertility (\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      fertilization).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Extender with 2% NL had the highest (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.001) total motility (69.85 \u00b1 0.92%), progressive motility (58.90 \u00b1 0.81%), sperm with integrated membrane (68.88 \u00b1 0.49%), sperm with active membrane (65.02 \u00b1 0.53%), sperm with intact acrosome (75.28 \u00b1 1.1%), and in an\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      fertilization system, the highest rates of cleavage (68.06 \u00b1 3.98%) and blastocyst formation (40.10 \u00b1 3.44%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion:<\/jats:title>\n                    <jats:p>NL provided better cryoprotection than NM for bull sperm. Supplementing the extender with NL could enhance bull semen cryopreservation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19475535261452091","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T15:38:41Z","timestamp":1782229121000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Adding Three Concentrations of Cold-Sensitive Nanoliposomes or Nanomicelles to Extender on Characteristics of Frozen\u2013Thawed Bull Sperm"],"prefix":"10.1177","author":[{"given":"Mahdieh Abbaszadeh","family":"Afshar","sequence":"first","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0186-1120","authenticated-orcid":false,"given":"Armin","family":"Towhidi","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Touba","family":"Nadri","sequence":"additional","affiliation":[{"name":"Department of Animal Science, College of Agriculture, Urmia University, Urmia, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdi","family":"Zhandi","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdollah Mohammadi","family":"Sangcheshmeh","sequence":"additional","affiliation":[{"name":"Department of Animal and Poultry Science, Faculty of Agricultural Technology, University College of Agriculture and Natural Resources, University of Tehran, Pakdasht, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Kastelic","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/19396368.2014.902521"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(92)90231-F"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00682-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.1997.tb01272.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1071\/RD04034"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.12.012"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2019.106171"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.04.024"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","unstructured":"Nagarajan R. 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