{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T18:09:21Z","timestamp":1789063761798,"version":"build-2803163510"},"reference-count":40,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"abstract":"<jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>\n                      Cryopreservation of semen is commonly linked to reduced sperm movement, compromised membrane integrity, and reduced functional capacity due to excessive production of reactive oxygen species (ROS).\n                      <jats:italic toggle=\"yes\">Moringa oleifera<\/jats:italic>\n                      leaf extract is recognized for its powerful antioxidant capabilities, which can neutralize ROS directly or boost the function of natural antioxidant enzymes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      This research was undertaken to assess the impact of incorporating moringa leaf extract into a TRIS-citrate-yolk extender on antioxidant enzyme activity, post-thaw sperm quality, and\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      fertility in Barbari bucks.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Semen samples were obtained from eight healthy bucks and divided into four treatment groups based on the concentration of moringa extract in the extender 0 mg\/100 mL (Group I), 50 mg\/100 mL (Group II), 100 mg\/100 mL (Group III), and 200 mg\/100 mL (Group IV). Following cryopreservation and thawing, the sperm samples were assessed for various quality parameters, antioxidant enzyme activity, and zona binding capacity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The results indicated that adding moringa extract significantly reduced (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.01) ROS generation and lipid peroxidation in all treatment groups compared with the control. Moreover, sperm motility, membrane integrity, and functional quality were better preserved in the extract-supplemented groups, with Group III showing the most notable improvement. The zona binding assay further confirmed enhanced sperm\u2013egg interaction in the treated groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The addition of moringa leaf extract to the extender effectively mitigates oxidative stress-related damage during cryopreservation and thawing, thereby improving sperm viability and fertility potential in Barbari buck semen. This method shows potential for enhancing reproductive outcomes in artificial insemination programs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19475535261475470","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:46:56Z","timestamp":1788889616000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Moringa Leaf Aqueous Extract as a Natural Antioxidant for Improving Cryopreserved Semen Quality and Fertility in Barbari Bucks"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0826-0811","authenticated-orcid":false,"given":"Chetna","family":"Gangwar","sequence":"first","affiliation":[{"name":"Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.D.","family":"Kharche","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division, ICAR-CIRG, Farah, Mathura, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shriprakash","family":"Singh","sequence":"additional","affiliation":[{"name":"Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshuman","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding, FVAS, Banaras Hindu University, Mirzapur, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd","family":"Arif","sequence":"additional","affiliation":[{"name":"Indian Institute of Farming Systems Research, Modipuram, Meerut, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juhi","family":"Pathak","sequence":"additional","affiliation":[{"name":"Animal Physiology and Reproduction Division, ICAR-CIRG, Farah, Mathura, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Pourouchottamane","sequence":"additional","affiliation":[{"name":"Animal Nutrition and Product Technology Division, ICAR-CIRG, Farah, Mathura, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhil","family":"Patel","sequence":"additional","affiliation":[{"name":"Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brijesh Kumar","family":"Yadav","sequence":"additional","affiliation":[{"name":"Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita","family":"Singh","sequence":"additional","affiliation":[{"name":"Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.5958\/0973-9718.2023.00017.X"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.153850"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.03.005"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/iju.IJU_9_17"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13020"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.05.012"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13322"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.604460"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.56093\/ijans.v94i4.146821"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.7439\/ijbar.v2i6.35"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/app13074387"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160612791"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2023"},{"issue":"49","key":"e_1_3_2_15_1","first-page":"8467","article-title":"Active principle from Moringa oleifera Lam leaves effective against two leukemias and a hepatocarcinoma","volume":"9","author":"Khalafalla MM","year":"2010","unstructured":"14.Khalafalla MM, , Abdellatef E, , Dafalla HM, et al. 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