{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:27:29Z","timestamp":1787646449903,"version":"build-2736575974"},"reference-count":28,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"The Open Research Fund of the State Key Laboratory of Digital Medical Engineering, Southeast University","award":["No. 2025-M04"],"award-info":[{"award-number":["No. 2025-M04"]}]},{"name":"CAMS Plastic Surgery Hospital Institutional Fund","award":["No. YSZ2024CG002"],"award-info":[{"award-number":["No. YSZ2024CG002"]}]},{"name":"CAMS Plastic Surgery Hospital Institutional Fund","award":["No.YS2024CG034"],"award-info":[{"award-number":["No.YS2024CG034"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>High-quality primary cells and well-annotated clinical data are essential for reproducible skin-on-a-chip (SoC) research. However, conventional biobanking workflows often lack SoC-oriented procedures for specimen acquisition, live-cell preservation, quality control, and traceability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>We established a specialized plastic surgery biobank incorporating standardized tissue procurement, cold-chain transport at 2\u00b0C\u20138\u00b0C with processing initiated within 2\u20136 hours, hierarchical preservation of tissues and live cells, and a unique identifier (UID)-linked multimodal digital platform. The biobank primarily archives skin tissue specimens, while a representative subset of specimens was processed for primary-cell isolation and quality-control evaluation. Post-thaw cell viability was assessed primarily by trypan blue exclusion, with flow cytometry used as an auxiliary method in representative batches. Passage-associated lineage-marker expression was evaluated by immunofluorescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The biobank currently archives 450 skin tissue specimens, of which samples from donors aged 0\u201314 years account for 63.8%. Among the representative cell-production batches evaluated, all available trypan blue image and counting records showed a mean post-thaw viability of 93.91 \u00b1 3.37% (range, 88.24%\u2013100.00%; coefficient of variation, 3.59%), with a cell count\u2013weighted viability of 93.78%. P5 keratinocyte and fibroblast cultures showed near-complete K14 and vimentin positivity, respectively, whereas melanocyte melanocyte melan-A\/melanoma antigen recognized by T-cells 1 (MLANA\/MART-1) positivity was high at P1 (80.72 \u00b1 11.67%) and decreased at P5 (38.41 \u00b1 4.50%; Welch\u2019s\n                      <jats:italic toggle=\"yes\">t-<\/jats:italic>\n                      test,\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      = 0.021). The operational digital platform supports UID-based sample management, sample-imaging association, storage management, and structured metadata export.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>This study presents a framework for establishing a fit-for-purpose and traceable specialized biobank and digital information platform to support plastic surgery SoC research. The current findings provide preliminary quality-control evidence for viable keratinocyte and fibroblast preparations and support the preferential use of early passage, marker-guided melanocyte preparations. Comprehensive functional SoC validation, longitudinal assessment of cellular stability, and melanocyte-specific preservation and release criteria require further investigation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19475535261479035","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T05:05:57Z","timestamp":1787375157000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Framework for Establishing a Specialized Biobank and Digital Information Platform for Skin-on-a-Chip Research in Plastic Surgery"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2719-8864","authenticated-orcid":false,"given":"Xiuhong","family":"Li","sequence":"first","affiliation":[{"name":"Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianxiao","family":"Wei","sequence":"additional","affiliation":[{"name":"Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China."},{"name":"Skin-on-a-Chip Translational Medicine Center, Joint Laboratory of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Southeast University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Clinical Data and Biobank Resource, Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China."},{"name":"Skin-on-a-Chip Translational Medicine Center, Joint Laboratory of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Southeast University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningbei","family":"Yin","sequence":"additional","affiliation":[{"name":"Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China."},{"name":"Skin-on-a-Chip Translational Medicine Center, Joint Laboratory of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Southeast University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuo","family":"Si","sequence":"additional","affiliation":[{"name":"Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China."},{"name":"Skin-on-a-Chip Translational Medicine Center, Joint Laboratory of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Southeast University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios14050225"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14030682"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering11111055"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms262110753"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.637048"},{"key":"e_1_3_2_7_1","unstructured":"6.State Administration for Market Regulation and Standardization Administration of China. GB\/T 44831-2024 General technical requirements for skin chips. Standards Press of China: Beijing; 2024. Available from: https:\/\/std.samr.gov.cn\/gb\/search\/gbDetailed?id=D43060CFC98A0672E05397BE0A0A52E6 [Last accessed: April8 2026]."},{"key":"e_1_3_2_8_1","unstructured":"7.International Organization for Standardization. ISO 20387:2018 Biotechnology: Biobanking: General requirements for biobanking. ISO: Geneva; 2018. Available from: https:\/\/www.iso.org\/standard\/67888.html [Last accessed: April8 2026]."},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.70759"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00246"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.14374"},{"issue":"1","key":"e_1_3_2_12_1","first-page":"17","article-title":"Organoid biobanks as a new tool for pre-clinical validation of candidate drug efficacy and safety","volume":"13","author":"Botti G","year":"2021","unstructured":"11.Botti G, , Di Bonito M, , Cantile M. Organoid biobanks as a new tool for pre-clinical validation of candidate drug efficacy and safety. 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