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Following imaging, the joint surfaces were excised, rinsed to remove contrast agent, and compressive stiffness (equilibrium and instantaneous compressive moduli) was measured via indentation testing (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 17 sites). Each site was sectioned for histology and assessed for glycosaminoglycan content using digital densitometry of Safranin-O stained sections, Fourier transform infrared spectroscopy for collagen content, and morphology using both the Mankin and OARSI semiquantitative scoring systems. Water content was determined using mass change after lyophilization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CECT attenuation at all imaging time points, including those &lt;1 hour of ioxaglate exposure, correlated significantly (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05) with cartilage water and glycosaminoglycan contents, Mankin score, and both equilibrium and instantaneous compressive moduli. Early time points (&lt;30 minutes) also correlated (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05) with collagen content and OARSI score. Differences in cartilage quality between intrajoint regions were distinguishable at diffusion equilibrium and after brief ioxaglate exposure.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CECT with ioxaglate affords biochemical and biomechanical measurements of cartilage health and performance even after short, clinically relevant exposure times, and may be useful in the clinic as a means for detecting early signs of cartilage pathology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1947603516665443","type":"journal-article","created":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T20:08:47Z","timestamp":1472846927000},"page":"391-399","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":19,"title":["Contrast-Enhanced Computed Tomography Enables Quantitative Evaluation of Tissue Properties at Intrajoint Regions in Cadaveric Knee Cartilage"],"prefix":"10.1177","volume":"8","author":[{"given":"Rachel C.","family":"Stewart","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Boston University, Boston, MA, USA"},{"name":"Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juuso T.J.","family":"Honkanen","sequence":"additional","affiliation":[{"name":"Department of Applied Physics, University of Eastern Finland, Kuopio, Finland"},{"name":"Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harri T.","family":"Kokkonen","sequence":"additional","affiliation":[{"name":"Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virpi","family":"Tiitu","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Anatomy, University of Eastern Finland, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simo","family":"Saarakkala","sequence":"additional","affiliation":[{"name":"Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland"},{"name":"Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland"},{"name":"Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antti","family":"Joukainen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Traumatology and Hand Surgery, Kuopio University Hospital, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian 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