{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:58:54Z","timestamp":1791824334801,"version":"4.3.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T00:00:00Z","timestamp":1480377600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["08\/56472-0"],"award-info":[{"award-number":["08\/56472-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["11\/51959-0"],"award-info":[{"award-number":["11\/51959-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["CARTILAGE"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Articular cartilage is an avascular tissue with limited ability of self-regeneration and the current clinical treatments have restricted capacity to restore damages induced by trauma or diseases. Therefore, new techniques are being tested for cartilage repair, using scaffolds and\/or stem cells. Although type II collagen hydrogel, fibrin sealant, and adipose-derived stem cells (ASCs) represent suitable alternatives for cartilage formation, their combination has not yet been investigated in vivo for focal articular cartilage defects. We performed a simple experimental procedure using the combination of these 3 compounds on cartilage lesions of rabbit knees.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>The hydrogel was developed in house and was first tested in vitro for chondrogenic differentiation. Next, implants were performed in chondral defects with or without ASCs and the degree of regeneration was macroscopically and microscopically evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Production of proteoglycans and the increased expression of collagen type II (COL2\u03b11), aggrecan (ACAN), and sex-determining region Y-box 9 (SOX9) confirmed the chondrogenic character of ASCs in the hydrogel in vitro. Importantly, the addition of ASC induced a higher overall repair of the chondral lesions and a better cellular organization and collagen fiber alignment compared with the same treatment without ASCs. This regenerating tissue also presented the expression of cartilage glycosaminoglycan and type II collagen.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results indicate that the combination of the 3 compounds is effective for articular cartilage repair and may be of future clinical interest.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1947603516675914","type":"journal-article","created":{"date-parts":[[2016,11,11]],"date-time":"2016-11-11T10:32:07Z","timestamp":1478860327000},"page":"439-443","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":20,"title":["Natural Type II Collagen Hydrogel, Fibrin Sealant, and Adipose-Derived Stem Cells as a Promising Combination for Articular Cartilage Repair"],"prefix":"10.1177","volume":"8","author":[{"given":"Mariana","family":"Lazarini","sequence":"first","affiliation":[{"name":"Hematology and Blood Transfusion Center, University of Campinas. National Institute of Blood Technology and Science, Campinas, S\u00e3o Paulo, Brazil"},{"name":"Department of Biological Sciences, Federal University of S\u00e3o Paulo, Diadema, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Bordeaux-Rego","sequence":"additional","affiliation":[{"name":"Hematology and Blood Transfusion Center, University of Campinas. National Institute of Blood Technology and Science, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Giardini-Rosa","sequence":"additional","affiliation":[{"name":"Hematology and Blood Transfusion Center, University of Campinas. National Institute of Blood Technology and Science, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana S. S.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Hematology and Blood Transfusion Center, University of Campinas. National Institute of Blood Technology and Science, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Ozello","family":"Baratti","sequence":"additional","affiliation":[{"name":"INFABiC, National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro Rozim","family":"Zorzi","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, University of Campinas, Campinas, S\u00e3o Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Batista","family":"de Miranda","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, University of Campinas, Campinas, S\u00e3o Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Lenz Cesar","sequence":"additional","affiliation":[{"name":"Biomedical Lasers Application Laboratory, Optics and Photonics Research Center, \u201cGleb Wataghin\u201d Institute of Physics, University of Campinas, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2ngela","family":"Luzo","sequence":"additional","affiliation":[{"name":"Hematology and Blood Transfusion Center, University of Campinas. National Institute of Blood Technology and Science, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Teresinha","family":"Olalla Saad","sequence":"additional","affiliation":[{"name":"Hematology and Blood Transfusion Center, University of Campinas. National Institute of Blood Technology and Science, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,11,29]]},"reference":[{"key":"bibr1-1947603516675914","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v3.i2.89"},{"key":"bibr2-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/438026"},{"key":"bibr3-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.12.011"},{"key":"bibr4-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.03.053"},{"key":"bibr5-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1117\/12.909185"},{"key":"bibr6-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2014.09.008"},{"key":"bibr7-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2007.05.005"},{"key":"bibr8-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.12.004"},{"key":"bibr9-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1159\/000147272"},{"key":"bibr10-1947603516675914","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc.2015.8.1.48"},{"key":"bibr11-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34896"},{"key":"bibr12-1947603516675914","doi-asserted-by":"publisher","DOI":"10.2174\/156652412800619950"},{"key":"bibr13-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0176"},{"key":"bibr14-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2007.0435"},{"key":"bibr15-1947603516675914","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.10.049"}],"container-title":["CARTILAGE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1947603516675914","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1947603516675914","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1947603516675914","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:01:13Z","timestamp":1777384873000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.1177\/1947603516675914"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,29]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1177\/1947603516675914"],"URL":"https:\/\/doi.org\/10.1177\/1947603516675914","relation":{},"ISSN":["1947-6035","1947-6043"],"issn-type":[{"value":"1947-6035","type":"print"},{"value":"1947-6043","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,29]]}}}