{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:59:36Z","timestamp":1792799976662,"version":"4.5.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"2_suppl","license":[{"start":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:00:00Z","timestamp":1566864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"European Union\u2019s Seventh Framework Program","award":["305815"],"award-info":[{"award-number":["305815"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["CARTILAGE"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Syndecan-4 plays a critical role in cartilage degradation during osteoarthritis (OA). The aim of this study was to investigate the expression and localization of syndecan-4 in different OA joint tissues.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>Syndecan-4 mRNA levels were quantified by reverse transcription\u2013polymerase chain reaction in human OA primary cells. Syndecan-4 was localized by immunohistochemistry in knee, hip, or shoulder OA bone\/cartilage biopsies. Syndecan-4 was quantified by immunoassay in chondrocytes culture supernatant and cell fraction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Using immunochemistry, syndecan-4 was observed in chondrocytes clusters in the superficial zone of OA knee, but not in OA hip or shoulder cartilage. No significant difference was detected in syndecan-4 expression level in sclerotic compared with nonsclerotic osteoblasts or in inflamed synoviocytes compared to normal\/reactive ones. Differentiated hypertrophic chondrocytes from knee, but not from hip cartilage, expressed more syndecan-4 than nonhypertrophic cells. Using an immunoassay for the extracellular domain of syndecan-4, we found 68% of the syndecan-4 in the culture supernatant of OA chondrocytes culture, suggesting that a large majority of the syndecan-4 is shed and released in the extracellular medium. The shedding rate was not affected by hypertrophic differentiation state of the chondrocytes or their joint origin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Even if chondrocytes clusters are seen in OA knee, hip and shoulder cartilage and hypertrophic differentiation appears in knee and hip OA articular chondrocytes, syndecan-4 synthesis only increased in knee. These findings suggest the presence of biochemical difference between articular cartilage according to their location and that syndecan-4 could be a biochemical marker specific for knee OA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1947603519870855","type":"journal-article","created":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T02:58:07Z","timestamp":1566961087000},"page":"862S-871S","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Syndecan-4 Is Increased in Osteoarthritic Knee, but Not Hip or Shoulder, Articular Hypertrophic Chondrocytes"],"prefix":"10.1177","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1005-8172","authenticated-orcid":false,"given":"Christelle","family":"Sanchez","sequence":"first","affiliation":[{"name":"Bone and Cartilage Research Unit, Arthrop\u00f4le Li\u00e8ge, University of Li\u00e8ge, Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Lambert","sequence":"additional","affiliation":[{"name":"Bone and Cartilage Research Unit, Arthrop\u00f4le Li\u00e8ge, University of Li\u00e8ge, Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Emile","family":"Dubuc","sequence":"additional","affiliation":[{"name":"Orthopedic Department, Cliniques Universitaires Saint-Luc, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Bertrand","sequence":"additional","affiliation":[{"name":"Experimental Orthopedics, University Hospital Magdeburg, Magdeburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Pap","sequence":"additional","affiliation":[{"name":"Institute of Experimental Musculoskeletal Medicine, University Hospital Munster, Munster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Henrotin","sequence":"additional","affiliation":[{"name":"Bone and Cartilage Research Unit, Arthrop\u00f4le Li\u00e8ge, University of Li\u00e8ge, Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,8,27]]},"reference":[{"key":"bibr1-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000159708.71142.c8"},{"key":"bibr2-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(98)00191-4"},{"key":"bibr3-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1053\/joca.1999.0286"},{"key":"bibr4-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.96B10.33812"},{"key":"bibr5-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1002\/art.37817"},{"key":"bibr6-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1998"},{"key":"bibr7-1947603519870855","doi-asserted-by":"publisher","DOI":"10.3109\/03008200903288902"},{"key":"bibr8-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.264549"},{"key":"bibr9-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23009"},{"key":"bibr10-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1002\/art.23159"},{"key":"bibr11-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1002\/art.33445"},{"key":"bibr12-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1002\/art.38315"},{"key":"bibr13-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1186\/ar3771"},{"key":"bibr14-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.01.010"},{"key":"bibr15-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1136\/ard.19.4.318"},{"key":"bibr16-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0194591"},{"key":"bibr17-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.03.008"},{"key":"bibr18-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/35.suppl_3.14"},{"key":"bibr19-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.07.014"},{"key":"bibr20-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2006.063875"},{"key":"bibr21-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-14-18"},{"key":"bibr22-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmrj.2012.01.020"},{"key":"bibr23-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0902639106"},{"key":"bibr24-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2015.02.058"},{"key":"bibr25-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1161\/hq0102.100314"},{"key":"bibr26-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.02.012"},{"key":"bibr27-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-009-9699-9"},{"key":"bibr28-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1002\/art.21215"},{"key":"bibr29-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.4.811"},{"key":"bibr30-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00548.2017"},{"key":"bibr31-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.23.14713"},{"key":"bibr32-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00093.2006"},{"key":"bibr33-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501903200"},{"key":"bibr34-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9045976"},{"key":"bibr35-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2506"},{"key":"bibr36-1947603519870855","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12174"}],"container-title":["CARTILAGE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1947603519870855","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1947603519870855","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1947603519870855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:01:54Z","timestamp":1777384914000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1947603519870855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,27]]},"references-count":36,"journal-issue":{"issue":"2_suppl","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1177\/1947603519870855"],"URL":"https:\/\/doi.org\/10.1177\/1947603519870855","relation":{},"ISSN":["1947-6035","1947-6043"],"issn-type":[{"value":"1947-6035","type":"print"},{"value":"1947-6043","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,27]]}}}