{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:45:46Z","timestamp":1790873146332,"version":"4.1.0"},"reference-count":39,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["CARTILAGE"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this study was to determine the matrix metalloproteinase-10 (MMP-10) expression pattern and to assess how it contributes to endochondral osteogenesis in Kashin-Beck disease (KBD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>The cartilages of KBD patients, Sprague-Dawley rats fed with selenium (Se)-deficient diet and\/or T-2 toxin, and ATDC5 cells were used in this study. ATDC5 cells were induced into hypertrophic chondrocytes using a 1% insulin-transferrin-selenium (ITS) culture medium for 21 days. The expressions of MMP-10 in the cartilages were visualized by immunohistochemistry. The messenger RNA (mRNA) and protein expression levels were determined by real-time polymerase chain reaction (RT-PCR) and Western blotting. MMP-10 short hairpin RNA (shRNA) was transfected into hypertrophic chondrocytes to knock down the gene expression of MMP-10. Meanwhile, the cell death of MMP-10-knockdown chondrocyte was detected using flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The expression of MMP-10 was decreased in the growth plates of children with KBD. A decreased expression of MMP-10 also was observed in the growth plates of rats fed with an Se-deficient diet and\/or T-2 toxin exposure. The mRNA and protein expression levels of MMP-10 increased during the chondrogenic differentiation of ATDC5 cells. MMP-10 knockdown in hypertrophic chondrocytes significantly decreased the gene and protein expression of collagen type II (Col II), Col X, Runx2, and MMP-13. Besides, the percentage of cell apoptosis was significantly increased after MMP-10 knockdown in hypertrophic chondrocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MMP-10 deficiency disrupts chondrocyte terminal differentiation and induces the chondrocyte\u2019s death, which impairs endochondral osteogenesis in the pathogenesis of KBD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19476035221109226","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:54:29Z","timestamp":1657587269000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["MMP-10 Deficiency Effects Differentiation and Death of Chondrocytes Associated with Endochondral Osteogenesis in an Endemic Osteoarthritis"],"prefix":"10.1177","volume":"13","author":[{"given":"Dan","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5597-2470","authenticated-orcid":false,"given":"Xingxing","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2807-7184","authenticated-orcid":false,"given":"Meng","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengfen","family":"Yi","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoru","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyou","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9824-3411","authenticated-orcid":false,"given":"Cuiyan","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghong","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center, Xi\u2019an Jiaotong University, Xian, China"},{"name":"Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People\u2019s Republic of China, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,7,11]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002640000190."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.07.023."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20652."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20236009."},{"issue":"1","key":"e_1_3_2_6_2","first-page":"19","article-title":"A study on abnormal chondrocyte differentiation and abnormal expression of collagen types in articular cartilage from patients with Kachin-Beck disease [in Chinese]","volume":"27","author":"Guo X","year":"1998","unstructured":"Guo X, Thomas A, Pirkko L. A study on abnormal chondrocyte differentiation and abnormal expression of collagen types in articular cartilage from patients with Kachin-Beck disease [in Chinese]. Zhonghua Bing Li Xue Za Zhi. 1998;27(1):19-22.","journal-title":"Zhonghua Bing Li Xue Za Zhi"},{"issue":"4","key":"e_1_3_2_7_2","first-page":"696","article-title":"Morphology and phenotype expression of types I, II, III, and X collagen and MMP-13 of chondrocytes cultured from articular cartilage of Kashin-Beck disease","volume":"35","author":"Wang W","year":"2008","unstructured":"Wang W, Guo X, Chen J, Xu P, Lammi MJ. Morphology and phenotype expression of types I, II, III, and X collagen and MMP-13 of chondrocytes cultured from articular cartilage of Kashin-Beck disease. J Rheumatol. 2008;35(4):696-702.","journal-title":"J Rheumatol"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.10.004."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.32607\/20758251-2014-6-3-89-97."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.2741\/1916."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.815400."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2008.10.046."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-004-0161-6."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.27512."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.4.639."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1553."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/s8756-3282(98)00064-7."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22167."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27766."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002640100242."},{"issue":"4","key":"e_1_3_2_21_2","article-title":"Determination of T-2 toxin in staple food from a Kashin-Beck disease (KBD) area and non-KBD areas in Heilongjiang Province [in Chinese]","volume":"16","author":"Sun DJ","year":"1997","unstructured":"Sun DJ, Liu QY, Li QW. Determination of T-2 toxin in staple food from a Kashin-Beck disease (KBD) area and non-KBD areas in Heilongjiang Province [in Chinese]. Chin J Epidem. 1997;16(4):207-9.","journal-title":"Chin J Epidem"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2007.03.013."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1756-185X.2010.01530.x."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/1947603518809406."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-006-0690-3."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24467."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2287."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24243."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.504."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200211089."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2014.444."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.105536."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920108783497659."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.27512."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073024."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/iep.12297."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.11.050."},{"issue":"3","key":"e_1_3_2_38_2","article-title":"Chondrocyte apoptosis and expression of Bcl-2, Bax, Fas, and iNOS in articular cartilage in patients with Kashin-Beck disease","volume":"33","author":"Wang SJ","year":"2006","unstructured":"Wang SJ, Guo X, Zuo H, Zhang YG, Xu P, Ping ZG, et al. Chondrocyte apoptosis and expression of Bcl-2, Bax, Fas, and iNOS in articular cartilage in patients with Kashin-Beck disease. J Rheumatol. 2006;33(3):615-9.","journal-title":"J Rheumatol"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2018.08.017."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1177\/1947603519886626."}],"container-title":["CARTILAGE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/19476035221109226","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/19476035221109226","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/19476035221109226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:59:23Z","timestamp":1777384763000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/19476035221109226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1177\/19476035221109226"],"URL":"https:\/\/doi.org\/10.1177\/19476035221109226","relation":{},"ISSN":["1947-6035","1947-6043"],"issn-type":[{"value":"1947-6035","type":"print"},{"value":"1947-6043","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7]]},"article-number":"19476035221109226"}}