{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:46:37Z","timestamp":1789494397808,"version":"build-2803163510"},"reference-count":33,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["CARTILAGE"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Articular cartilage defect causes joint pain and finally progresses to osteoarthritis. Although the subchondral bone condition affects clinical outcomes of cartilage defects, the natural course of changes in subchondral bone and associated pain in full-thickness cartilage defects remain unknown. Therefore, we investigated the natural course of histological changes in subchondral bone and joint pain in cartilage defects using a rat model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>Full-thickness cartilage defects were created at the medial femoral condyle of 10-week-old male Sprague-Dawley rats. Rats were sacrificed at 3, 7, 14, 28, and 56 days postoperatively, and histological including immunohistochemistry and tartrate-resistant acid phosphatase (TRAP) staining and micro-computed tomography (\u03bcCT) analyses of their knees were performed. Pain was evaluated using behavioral analysis and immunofluorescence staining of the dorsal root ganglion (DRG).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The contour of the subchondral bone plate was maintained until day 3, but it was absorbed just under the cartilage defect from day 7 to 14. Starting on day 28, sclerotic changes surrounding the bone absorption area were detected. In the subchondral bone, the number of TRAP-positive cells peaked on day 14. Osteocalcin-positive cells were observed at 7 days, and their number gradually increased till day 56. Behavioral analysis showed that the total distance and the number of getting up by hind legs decreased on day 14. The number of calcitonin gene-related peptide-positive fibers in the DRG increased and was the highest on day 14.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The subchondral bone condition under cartilage defects dynamically changes from bone resorption to sclerosis and is related to pain level.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19476035231154514","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T00:02:50Z","timestamp":1676419370000},"page":"247-255","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Changes in the Subchondral Bone Affect Pain in the Natural Course of Traumatic Articular Cartilage Defects"],"prefix":"10.1177","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1583-3884","authenticated-orcid":false,"given":"Yuichi","family":"Kato","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Nakasa","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"},{"name":"Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Sumii","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munekazu","family":"Kanemitsu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Artificial Joints and Biomaterials, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Miyaki","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"},{"name":"Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Adachi","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,2,14]]},"reference":[{"key":"bibr1-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.07.013"},{"key":"bibr2-19476035231154514","doi-asserted-by":"publisher","DOI":"10.5435\/00124635-201005000-00002"},{"key":"bibr3-19476035231154514","volume":"47","author":"Buckwalter JA","year":"1998","journal-title":"Instr Course Lect"},{"key":"bibr4-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1038\/ncprheum0676"},{"key":"bibr5-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1177\/1947603521993219"},{"key":"bibr6-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1053\/jars.2003.50112"},{"key":"bibr7-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1177\/0363546508330137"},{"key":"bibr8-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1177\/0363546518787287"},{"key":"bibr9-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2003.09.016"},{"key":"bibr10-19476035231154514","volume":"59","author":"van Dijk CN","year":"2010","journal-title":"Instr Course Lect"},{"key":"bibr11-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077824"},{"key":"bibr12-19476035231154514","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.66532"},{"key":"bibr13-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-006-0277-5"},{"key":"bibr14-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1002\/art.27713"},{"key":"bibr15-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-018-2221-5"},{"key":"bibr16-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173726"},{"key":"bibr17-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.02.004"},{"key":"bibr18-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2014.01.014"},{"issue":"1","key":"bibr19-19476035231154514","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0891-8422(23)01166-7","volume":"18","author":"Hayes DW","year":"2001","journal-title":"Clin Podiatr Med Surg"},{"key":"bibr20-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-010-1064-x"},{"key":"bibr21-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2012.217"},{"key":"bibr22-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.131904"},{"key":"bibr23-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1172\/JCI121561"},{"key":"bibr24-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kel213"},{"key":"bibr25-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1186\/ar1716"},{"key":"bibr26-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2009.08.004"},{"key":"bibr27-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1002\/art.30307"},{"key":"bibr28-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1053\/joca.1999.0241"},{"key":"bibr29-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-198612000-00005"},{"key":"bibr30-19476035231154514","volume":"8","author":"Yang Y","year":"2020","journal-title":"PeerJ"},{"key":"bibr31-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1002\/art.27595"},{"key":"bibr32-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2011-200970"},{"key":"bibr33-19476035231154514","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650071410"}],"container-title":["CARTILAGE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/19476035231154514","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/19476035231154514","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/19476035231154514","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:03:00Z","timestamp":1777384980000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/19476035231154514"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,14]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1177\/19476035231154514"],"URL":"https:\/\/doi.org\/10.1177\/19476035231154514","relation":{},"ISSN":["1947-6035","1947-6043"],"issn-type":[{"value":"1947-6035","type":"print"},{"value":"1947-6043","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,14]]}}}