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Passage 2 (P2) JCC sheets from all donors were transplanted into nude rat chondral defects for 4 weeks (27 nude rats in total). Defect-only group served as control. Histological samples were stained for safranin O, collagen 1 (COL1), and collagen 2 (COL2). (1) All samples were scored, and correlation coefficients for each score were calculated. (2) Donors were divided into \u201cmore effective\u201d and \u201cless effective\u201d groups based on these scores. Then, differences between each group in each category of modified O\u2019Driscoll scoring were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>(1) Modified O\u2019Driscoll scores were negatively correlated with %COL1 area, and positively correlated with %COL2 area and COL2\/1 ratio. (2) Four of 8 donors exhibited significantly higher modified O\u2019Driscoll scores and %COL2 areas. JCC donors were divided into two groups by average score values. Significant differences between the two groups were observed in modified O\u2019Driscoll categories of \u201cNature of predominant tissue,\u201d \u201cReconstruction of subchondral bone,\u201d and \u201cSafranin O staining.\u201d<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The combined histological evaluation method is useful for detailed\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      efficacy assessments of cartilage defect regeneration models. Variations in histological scores among juvenile cartilage-derived chondrocyte donors were correlated to the quality of regenerated cartilage hyaline structure and subchondral bone remodeling observed in the nude rat defect model.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19476035241277946","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T13:39:45Z","timestamp":1727357985000},"page":"885-897","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Regenerative Variability of Human Juvenile Chondrocyte Sheets From Different Cell Donors in an Athymic Rat Knee Chondral Defect Model"],"prefix":"10.1177","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5296-0502","authenticated-orcid":false,"given":"Keisuke","family":"Matsukura","sequence":"first","affiliation":[{"name":"Cell Sheet Tissue Engineering Center, Department of 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