{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:42:07Z","timestamp":1792795327873,"version":"4.5.0"},"reference-count":35,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Shanghai Municipal Hospital Orthopedic Specialist Alliance, Shanghai Municipal Health Commission key priority discipline project; Shanghai Spinal Disease and Trauma Orthopedics Research Center","award":["2022ZZ01014"],"award-info":[{"award-number":["2022ZZ01014"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81820108020"],"award-info":[{"award-number":["81820108020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002339"],"award-info":[{"award-number":["82002339"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172461"],"award-info":[{"award-number":["82172461"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Frontiers Science Center of Degeneration and Regeneration in Skeletal System","award":["BJ1-9000-22-4002"],"award-info":[{"award-number":["BJ1-9000-22-4002"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["CARTILAGE"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Cellular senescence, i.e., a state of permanent cessation of cell division, is a hallmark of aging and has been associated with age-related diseases, most notably osteoarthritis (OA). Here we assessed senescence in chondrocytes, first\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      after treatment with the mutagens N-ethyl-N-nitrosourea (ENU) or bleomycin, and then in\n                      <jats:italic toggle=\"yes\">vivo<\/jats:italic>\n                      in cartilage samples from OA patients and control subjects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Cellular senescence in cultured chondrocytes treated with mutagens was assessed by senescence-associated \u03b2-galactosidase (SA-\u03b2-gal) staining and by evaluating the expression levels of\n                      <jats:italic toggle=\"yes\">p16<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">p21<\/jats:italic>\n                      . Cellular senescence in human hip cartilage chondrocytes from OA patients or non-OA controls was similarly evaluated. Apoptosis\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      was measured by the\n                      <jats:italic toggle=\"yes\">Bcl-2<\/jats:italic>\n                      \/\n                      <jats:italic toggle=\"yes\">Bax<\/jats:italic>\n                      expression ratio, and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      by both TUNEL assay and the\n                      <jats:italic toggle=\"yes\">Bcl-2<\/jats:italic>\n                      \/\n                      <jats:italic toggle=\"yes\">Bax<\/jats:italic>\n                      ratio.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In human articular cartilage, senescent cells were found to be significantly elevated in OA lesions of patients as compared with normal cartilage of non-OA control subjects.\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      , senescence was observed in bleomycin-treated chondrocytes, but not in ENU-treated cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings demonstrate that cellular senescence is associated with the pathogenesis of OA, with DNA damage and mutations as potential contributing factors in OA-associated senescence.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19476035251344875","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T02:32:46Z","timestamp":1750818766000},"page":"762-770","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Cellular Senescence in Human Chondrocytes in Relation to Osteoarthritis"],"prefix":"10.1177","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5327-0948","authenticated-orcid":false,"given":"Yu","family":"Qiang","sequence":"first","affiliation":[{"name":"Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9885-5372","authenticated-orcid":false,"given":"Chen","family":"Zheng","sequence":"additional","affiliation":[{"name":"Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander Y.","family":"Maslov","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Lu","sequence":"additional","affiliation":[{"name":"Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Shanghai Sixth People\u2019s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6082-945X","authenticated-orcid":false,"given":"Peijun","family":"Ren","sequence":"additional","affiliation":[{"name":"Center for Single-Cell Omics, School of Public Health, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2286-1069","authenticated-orcid":false,"given":"Yidan","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Shanghai Sixth People\u2019s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Vijg","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,16]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(19)30417-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.65"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.11.001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.10.005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1257"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature16932"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.015"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12587"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-121808-102144"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9363"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-00314-w"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2021-221513"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.41312"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00691-3"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09491-5"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-020-00533-7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.52"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2017.11.001"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-022-02859-x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa5612"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2892"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4324"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.03.001"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fragi.2022.1018119"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29325-6"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13698"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2021.11.009"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","unstructured":"Ren P Zheng C Pang Y Sun S Wang Q Xu W et al. Single-cell analysis of the somatic mutational landscape in human chondrocytes during aging and in osteoarthritis. bioRxiv:2024.08.24.609506 [Preprint]. 2024. doi:10.1101\/2024.08.24.609506%J.","DOI":"10.1101\/2024.08.24.609506%J"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12562"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072358"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2024.10.299"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rdc.2004.04.002"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.822816"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201439245"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.42822"}],"container-title":["CARTILAGE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/19476035251344875","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/19476035251344875","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/19476035251344875","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T11:54:37Z","timestamp":1786708477000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/19476035251344875"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,16]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1177\/19476035251344875"],"URL":"https:\/\/doi.org\/10.1177\/19476035251344875","relation":{},"ISSN":["1947-6035","1947-6043"],"issn-type":[{"value":"1947-6035","type":"print"},{"value":"1947-6043","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,16]]}}}