{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T14:28:11Z","timestamp":1787927291286,"version":"build-2784847793"},"reference-count":38,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100010270","name":"Capital Health Research and Development of Special Fund","doi-asserted-by":"publisher","award":["No. 2022-2-7033"],"award-info":[{"award-number":["No. 2022-2-7033"]}],"id":[{"id":"10.13039\/501100010270","id-type":"DOI","asserted-by":"publisher"}]},{"name":"New Drug Research and Development Incubation Project of the Beijing University of Chinese Medicine Third Affiliated Hospital","award":["No. BZYSY-2022-XYYF-22"],"award-info":[{"award-number":["No. BZYSY-2022-XYYF-22"]}]},{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["No. 7232026"],"award-info":[{"award-number":["No. 7232026"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["CARTILAGE"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Post-traumatic ankle osteoarthritis (TAA) is driven by injury-related inflammation, cartilage matrix loss, and chondrocyte phenotypic instability. Indian hedgehog (IHH) is implicated in hypertrophic and catabolic cartilage remodeling, but whether local miR-199a-5p administration is associated with changes in IHH-related expression patterns in traumatic ankle cartilage remains incompletely defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Forty-eight male Sprague-Dawley rats were randomized to sham, TAA model, miR-199a-5p agomir, or negative-control agomir groups. TAA-like cartilage degeneration was induced by medial malleolar fracture followed by unrestricted cage activity. From week 2, rats received weekly intra-articular saline, miR-199a-5p agomir, or negative-control agomir for six weeks. Longitudinal inflammation, function, and ankle circumference ratio were assessed, followed by radiography, histology, OARSI scoring, ELISA, Western blotting, and RT-qPCR at week 8.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The fracture model caused persistent swelling and functional impairment, radiographic post-traumatic changes, cartilage surface disruption, proteoglycan depletion, increased IL-1\u03b2\/TNF-\u03b1 production, increased IHH\/RUNX2\/MMP-13 expression, and reduced SOX9\/COL2A1 expression. miR-199a-5p agomir treatment partially improved function, reduced inflammatory scores and cytokine levels, lowered OARSI scores, and was associated with lower IHH expression and a less hypertrophic\/catabolic marker profile. The negative-control agomir showed no comparable protective pattern.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Intra-articular miR-199a-5p agomir attenuated experimental TAA-like cartilage degeneration. The protective phenotype was associated with reduced inflammation, lower IHH expression, attenuation of hypertrophic\/catabolic marker expression, and partial restoration of cartilage matrix-related markers. Direct miR-199a-5p binding to IHH, downstream GLI activation, and local miRNA tissue accumulation were not tested and require further study.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/19476035261482024","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T14:16:12Z","timestamp":1787580972000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Intra-Articular miR-199a-5p Attenuates Post-traumatic Ankle Cartilage Degeneration With Reduced IHH Expression and Hypertrophic-Catabolic Marker Expression"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8108-000X","authenticated-orcid":false,"given":"Zhihong","family":"Fu","sequence":"first","affiliation":[{"name":"Third Affiliated Hospital of Beijing University of Chinese Medicine"},{"name":"Beijing University of Chinese 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