{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:35:48Z","timestamp":1777851348101,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[1972,8,1]],"date-time":"1972-08-01T00:00:00Z","timestamp":81475200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[1972,8]]},"abstract":"<jats:p>The relationship between cytochrome oxidase activity assayed biochemically with 3,3'-diaminobenzidine (DAB) as substrate and the resulting electron microscopic staining reaction has been investigated. Mitochondria isolated from both fresh and perfusion-fixed (1% glutaraldehyde and phosphate buffer for 1-2 min) rat liver showed a significant increase in oxygen consumption, as measured by an oxygen electrode, after addition of DAB and were further stimulated by adding cytochrome c. 2,4-Dinitrophenol and antimycin had no effect on the oxidation of DAB; cyanide and azide completely inhibited DAB oxidation, resulting in the absence of any mitochondrial staining reaction. Already after incubation for only 2 min mitochondria exhibited electron-dense deposits on the surface of the inner mitochondrial membrane away from the matrix. The results indicate that DAB donates electrons at the cytochrome c level to the electron transfer system of the respiratory chain and demonstrate the direct relationship between the electron microscopic staining reaction and cytochrome oxidase activity.<\/jats:p>","DOI":"10.1177\/20.8.583","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T01:48:12Z","timestamp":1301622492000},"page":"583-589","source":"Crossref","is-referenced-by-count":32,"title":["DEMONSTRATION OF CYTOCHROME OXIDASE ACTIVITY WITH DIAMINOBENZIDINE A BIOCHEMICAL AND ELECTRON MICROSCOPIC STUDY"],"prefix":"10.1177","volume":"20","author":[{"given":"ALBRECHT","family":"REITH","sequence":"first","affiliation":[{"name":"FB Physik, Universit\u00e4t Konstanz, D-7750 Konstanz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BARBARA","family":"SCH\u00dcLER","sequence":"additional","affiliation":[{"name":"FB Physik, Universit\u00e4t Konstanz, D-7750 Konstanz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1972,8,1]]},"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/20.8.583","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/20.8.583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:20:01Z","timestamp":1777501201000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/20.8.583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,8]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1972,8]]}},"alternative-id":["10.1177\/20.8.583"],"URL":"https:\/\/doi.org\/10.1177\/20.8.583","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,8]]}}}