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Outbreaks of keratoconjunctivitis are problematic to human health, especially for infants, the elderly, and immunocompromised individuals. However, the development of anti-HAdV drugs has been hampered by inconvenient screening systems; therefore, development of a simple screening method is highly desirable. In this study, we identified that HAdV-19 can infect a human lymphoid cell line transformed with human T-cell leukemia virus (MT-2 cells). MT-2 cells supported HAdV-19 replication and showed apparent cytopathic effects within five\u2009days post-infection. Using a thiazolyl blue tetrazolium bromide (MTT)-based colorimetric assay on MT-2 cells, we were able to detect the anti-HAdV-19 activities of previously reported nucleoside\/tide compounds, including (S)-1\u2013(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (cidofovir), 2\u2032,3\u2032-dideoxycytidine (zalcitabine) and 3\u2032-deoxy-3\u2032-fluorothymidine (trifluridine). Compared with previous methods, this system represents a more simple and rapid method to screen anti-HAdV-19 agents.<\/jats:p>","DOI":"10.1177\/2040206620921319","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T01:25:32Z","timestamp":1588123532000},"page":"204020662092131","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Application of human lymphoid cells for the evaluation of antivirals against human adenovirus type 19: Zalcitabine has superior activity compared to cidofovir"],"prefix":"10.1177","volume":"28","author":[{"given":"Kohsuke","family":"Nakagawara","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, International Institute of Disaster Science, Graduate School of Medicine, Tohoku Medical Megabank Organization, Tohoku University, Miyagi, Japan"},{"name":"Biochemicals Division, Yamasa Corporation, Choshi, 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