{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:39:57Z","timestamp":1753882797508,"version":"3.41.2"},"reference-count":9,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antivir Chem Chemother"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p> Human norovirus is the main cause of viral gastroenteritis, resulting annually in\u2009\u223c\u2009700 million infections and 200,000 deaths, of whom most are children &lt;5\u2009years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norovirus-targeting small molecules. We have previously established antiviral assays using this system, identified novel inhibitors and performed additional studies in order to have a first insight into their mechanism of action. After the identification of novel small molecules with anti-norovirus activity (part 1 of this protocol), we here describe the logical next step which entails the generation of early information of their mode of action. This information together with a continuous improvement of the potency of compounds will contribute to the optimization of a compound class towards in vivo efficacy and a successful preclinical development. <\/jats:p>","DOI":"10.1177\/20402066211025175","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T07:45:51Z","timestamp":1631778351000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies"],"prefix":"10.1177","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1632-3499","authenticated-orcid":false,"given":"Jana Van","family":"Dycke","sequence":"first","affiliation":[{"name":"KU Leuven \u2013 Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3838-310X","authenticated-orcid":false,"given":"Jasper","family":"Rymenants","sequence":"additional","affiliation":[{"name":"KU Leuven \u2013 Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Neyts","sequence":"additional","affiliation":[{"name":"KU Leuven \u2013 Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joana","family":"Rocha-Pereira","sequence":"additional","affiliation":[{"name":"KU Leuven \u2013 Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"key":"bibr1-20402066211025175","doi-asserted-by":"crossref","unstructured":"Dycke et al. Assessment of the antinorovirus activity in cell culture using the mouse norovirus: identification of active compounds. Antivir Chem Chemother (In press). 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