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Using stem cells, a patient\u2019s autologous cells, or allografted cells to seed-engineered scaffolds, tissue-engineered constructs can effectively supplement the donor pool and bypass other problems that arise when using donor organs, such as who receives the organ first and whether donor organ rejection may occur. However, current research methods and technologies have been unable to successfully engineer and vascularize large volume tissue constructs. This review examines the current perfusion methods for ex vivo organ systems, defines the different types of vascularization in organs, explores various strategies to vascularize ex vivo organ systems, and discusses challenges and opportunities for the field of tissue engineering.<\/jats:p>","DOI":"10.1177\/2041731418772949","type":"journal-article","created":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T10:55:01Z","timestamp":1525949701000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Engineering a microcirculation for perfusion control of ex vivo\u2013assembled organ systems: Challenges and opportunities"],"prefix":"10.1177","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6186-3676","authenticated-orcid":false,"given":"Pavan","family":"Kottamasu","sequence":"first","affiliation":[{"name":"Tufts University School of Medicine, Boston, MA, 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