{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T07:12:59Z","timestamp":1777878779772,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000900","name":"California Institute for Regenerative Medicine","doi-asserted-by":"publisher","award":["CIRMTRAN1-08471"],"award-info":[{"award-number":["CIRMTRAN1-08471"]}],"id":[{"id":"10.13039\/100000900","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Tissue Eng"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Models for enteric neuropathies, in which intestinal nerves are absent or injured, are required to evaluate possible cell therapies. However, existing options, including transgenic mice, are variable and fragile. Here immunocompromised mice were implanted with human pluripotent stem cell\u2013derived tissue-engineered small intestine 10\u2009weeks prior to a second survival surgery in which enteric nervous system precursor cells, or saline controls, were injected into the human intestinal organoid\u2013derived tissue-engineered small intestine and analyzed 4\u2009weeks later. Human intestinal organoid\u2013derived tissue-engineered small intestine implants injected with saline as controls illustrated formation of intestinal epithelium and mesenchyme without an enteric nervous system. Second surgical introduction of human pluripotent stem cell\u2013generated enteric nervous system precursors into developing human intestinal organoid\u2013derived tissue-engineered small intestine implants resulted in proliferative migratory neuronal and glial cells, including multiple neuronal subtypes, and demonstrated function in contractility assays.<\/jats:p>","DOI":"10.1177\/2041731420905701","type":"journal-article","created":{"date-parts":[[2020,4,27]],"date-time":"2020-04-27T14:49:08Z","timestamp":1587998948000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Induced pluripotent stem cell-derived enteric neural crest cells repopulate human aganglionic tissue-engineered intestine to form key components of the enteric nervous system"],"prefix":"10.1177","volume":"11","author":[{"given":"David F","family":"Chang","sequence":"first","affiliation":[{"name":"Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel M","family":"Zuber","sequence":"additional","affiliation":[{"name":"Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A","family":"Gilliam","sequence":"additional","affiliation":[{"name":"Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura-Marie A","family":"Nucho","sequence":"additional","affiliation":[{"name":"Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Levin","sequence":"additional","affiliation":[{"name":"Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengnan","family":"Wang","sequence":"additional","affiliation":[{"name":"Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony I","family":"Squillaro","sequence":"additional","affiliation":[{"name":"Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sha","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Medical School, University of Michigan, Ann Arbor, MI, USA"},{"name":"Department of Cell & Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason R","family":"Spence","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Medical School, University of Michigan, Ann Arbor, MI, USA"},{"name":"Department of Cell & Developmental Biology, Medical School, University of Michigan, Ann Arbor, MI, USA"},{"name":"Program of Cellular & Molecular Biology, Medical School, University of Michigan, Ann Arbor, MI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy C","family":"Grikscheit","sequence":"additional","affiliation":[{"name":"Developmental Biology and Regenerative Medicine Program, The Saban Research Institute, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"},{"name":"Department of Surgery, Division of Pediatric Surgery, Children\u2019s Hospital Los Angeles, Los Angeles, CA, USA"},{"name":"Keck Medical School, University of Southern California, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,4,27]]},"reference":[{"key":"bibr1-2041731420905701","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2007.053959"},{"key":"bibr2-2041731420905701","unstructured":"Chen SH. https:\/\/www.sages.org\/wiki\/hirschsprung-disease\/ (accessed April 21 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