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CTG is designed to recapitulate the 3D extracellular matrix of the trachea with stable mechanical properties imparted from the extraluminal airway splint. We performed segmental orthotopic tracheal replacement in a mouse microsurgical model. MicroCT was used to measure graft patency. Tracheal neotissue formation was quantified histologically. Airflow dynamic properties were analyzed using computational fluid dynamics. We found that CTG are easily implanted and did not result in vascular erosion, tracheal injury, or inflammation. Graft epithelialization and endothelialization were comparable with CTG to control. Tracheal collapse was absent with CTG. Composite tracheal scaffolds combine biocompatible synthetic support with PDTG, supporting the regeneration of host epithelium while maintaining graft structure.<\/jats:p>","DOI":"10.1177\/20417314221108791","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T02:06:43Z","timestamp":1656295603000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":34,"title":["Tissue-engineered composite tracheal grafts create mechanically stable and biocompatible airway replacements"],"prefix":"10.1177","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9849-6881","authenticated-orcid":false,"given":"Lumei","family":"Liu","sequence":"first","affiliation":[{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children\u2019s Hospital, Columbus, OH, 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