{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:54:15Z","timestamp":1790812455582,"version":"4.1.0"},"reference-count":57,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"German Society for Lymphology","award":["Fo0315"],"award-info":[{"award-number":["Fo0315"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Tissue Eng"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>\n                    Chronic lymphedema after cancer treatment is common and there is still no cure for this disease. We herein investigated the lymphangiogenic capacity of adipose tissue-derived microvascular fragments (MVF), which contain stem cells and lymphatic vessel fragments. Secondary lymphedema was induced in the hindlimbs of C57BL\/6J mice. Green fluorescence protein (GFP)\n                    <jats:sup>+<\/jats:sup>\n                    MVF were isolated from transgenic C57BL\/6Tg (CAG-EGFP)1Osb\/J mice, suspended in collagen hydrogel, and injected in the lymphadenectomy defect of wild-type animals. This crossover model allowed the detection of MVF-derived blood and lymphatic vessels after transplantation. The MVF group was compared with animals receiving collagen hydrogel only or a sham intervention. Lymphangiogenic effects were analyzed using volumetry, magnetic resonance (MR) lymphography, histology, and immunohistochemistry. MVF injection resulted in reduced hindlimb volumes when compared to non-treated controls. MR lymphography revealed lymphatic regeneration with reduced dermal backflow after MVF treatment. Finally, MVF transplantation promoted popliteal angiogenesis and lymphangiogenesis associated with a significantly increased microvessel and lymphatic vessel density. These findings indicate that MVF transplantation represents a promising approach to induce therapeutic lymphangiogenesis.\n                  <\/jats:p>","DOI":"10.1177\/20417314221109957","type":"journal-article","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T08:16:21Z","timestamp":1658823381000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model"],"prefix":"10.1177","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0904-9591","authenticated-orcid":false,"given":"Florian S","family":"Frueh","sequence":"first","affiliation":[{"name":"Institute for Clinical and Experimental Surgery, Saarland University, Homburg\/Saar, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Gassert","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Surgery, Saarland University, Homburg\/Saar, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Scheuer","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental Surgery, Saarland University, Homburg\/Saar, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Clinic of Diagnostic and Interventional Radiology, Saarland University Medical Center, Homburg\/Saar, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Fries","sequence":"additional","affiliation":[{"name":"Clinic of Diagnostic and Interventional Radiology, Saarland University Medical Center, Homburg\/Saar, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne S","family":"Boewe","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Experimental 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