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To address this, we developed a human tubuloid-on-a-chip model integrating tubuloids, endothelial cells, and immune cells within a microfluidic system to recapitulate the key pathophysiology of renal fibrosis. Induction of fibrosis with TGF-\u03b21 in this system recapitulated key pathological features, including extracellular matrix deposition, epithelial-mesenchymal transition, and loss of epithelial polarity. Functional assessments revealed impaired tubular reabsorption, including reduced albumin uptake and glucose transport, alongside elevated oxidative stress, mirroring clinical observations in CKD patients. The model\u2019s pharmacological relevance was validated by the therapeutic effects of nintedanib, which attenuated fibrotic phenotypes. 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