{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T07:32:44Z","timestamp":1777879964804,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Therapeutic Advances in Infection"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background and objectives:<\/jats:title>\n                    <jats:p>Commercial Aspergillus IgG antibody assays have become pivotal in the current diagnosis of chronic pulmonary aspergillosis (CPA). However, diagnostic cutoffs have been found to vary from manufactures\u2019 recommendations in different settings. This study aimed to establish the Aspergillus IgG reference range among Nigerians and determine a diagnostic cutoff for CPA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Sera from 519 prospectively recruited healthy blood donors and 39 previously confirmed cases of CPA were analysed for Aspergillus IgG levels using the Bordier test kit (Bordier Affinity Products SA, Crissier, Switzerland). Accuracy versus cutoff profile and receiver operating characteristics (ROC) curve were analysed for both CPA cases and controls using the R-Studio (2020), (Window desktop, version 4.0.2 software with R packages \u201cnnet\u201d and \u201cROCR\u201d).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Among healthy blood donors, 141 (27.2%) were aged 16\u201325 years with median (interquartile range, IQR) of 22 (20\u201324) years; 304 (58.6%) were aged 26\u201340 years with median (IQR) of 32 (29\u201336) years; while 74 (14.2%) were aged 41\u201360 years with median (IQR) of 46 (44\u201349.75). Median IgG level in respective age groups were 0.069 (0.009\u20130.181), 0.044 (0.014\u20130.202) and 0.056 (0.01\u20130.265) with no significant difference found in the three age categories ( p\u2009=\u20090.69). The overall diagnostic cutoff for the diagnosis of CPA was 0.821 with an accuracy of 97.1% and area under the curve (AUC)\u2009=\u20090.986.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The optimal diagnostic cutoff for diagnosing CPA in Nigerians using the Bordier kit was 0.821 which is lower than the manufacturer\u2019s recommended cutoff of 1.0. The determination of this cutoff among Nigerians will significantly enhance accurate identification of CPA and assessment of its true burden in Nigeria.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/20499361211050158","type":"journal-article","created":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T18:58:26Z","timestamp":1633892306000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Standardization of\n                    <i>Aspergillus<\/i>\n                    IgG diagnostic cutoff in Nigerians"],"prefix":"10.1177","volume":"8","author":[{"given":"Rita O.","family":"Oladele","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology & Parasitology, College of Medicine, University of Lagos, PMB 12003, Lagos, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6009-2707","authenticated-orcid":false,"given":"Akaninyene A.","family":"Otu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Calabar, Calabar, Nigeria"},{"name":"Department of Infection and Travel Medicine, Leeds Teaching Hospitals NHS Trust, Leeds, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oluwaseyi J.","family":"Balogun","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Medicine, University of Lagos, Lagos, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4106-3035","authenticated-orcid":false,"given":"Oladayo M.","family":"Babalola","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Augustina O.","family":"Nwosu","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8111-0743","authenticated-orcid":false,"given":"Iriagbonse","family":"Iyabo Osaigbovo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, College of Medical Sciences, University of Benin, Benin City, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Titilayo","family":"Gbajabiamila","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, Lagos, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas K.","family":"Irurhe","sequence":"additional","affiliation":[{"name":"Department of Radiology, College of Medicine, University of Lagos, Lagos, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel A.","family":"Fayemiwo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology & Parasitology, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuwaram A.","family":"Shettima","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Parasitology and Immunology, Federal Medical Centre, Yola, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1059-5637","authenticated-orcid":false,"given":"Nkolika Stella","family":"Uwaezuoke","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Federal Medical Centre, Abuja, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chinagozi Precious","family":"Edwin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Aminu Kano Teaching Hospital, Kano, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyese Stephen","family":"Ayanbeku","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Federal Medical Centre, Bida, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joy U.","family":"Okaa","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2721-2903","authenticated-orcid":false,"given":"Charles John","family":"Elikwu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Benjamin Carson College of Medicine, Babcock University, Ilishan-Remo, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David W.","family":"Denning","sequence":"additional","affiliation":[{"name":"National Aspergillosis Centre, Manchester University NHS Foundation Trust, Manchester, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phyllis J.","family":"Kanki","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T.H Chan School of Public Health, Harvard University, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Folasade T.","family":"Ogunsola","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology & Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,10,9]]},"reference":[{"key":"bibr1-20499361211050158","doi-asserted-by":"publisher","DOI":"10.1378\/chest.121.6.1988"},{"key":"bibr2-20499361211050158","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1295716"},{"key":"bibr3-20499361211050158","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myv020"},{"key":"bibr4-20499361211050158","unstructured":"Leading International Fungal Education. 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