{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:30:21Z","timestamp":1791592221762,"version":"4.3.4"},"reference-count":28,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Therapeutic Advances in Infection"],"published-print":{"date-parts":[[2025,7]]},"abstract":"\n Background:<\/jats:title>\n \n Panton-Valentine Leukocidin (PVL) is one of the major virulence factors known to be associated with invasive, life-threatening\n Staphylococcus aureus<\/jats:italic>\n (\n S. aureus<\/jats:italic>\n ) soft tissue infections. Several studies have shown that methicillin-resistant\n S. aureus<\/jats:italic>\n (MRSA) and methicillin-susceptible\n S. aureus<\/jats:italic>\n (MSSA) are carriers of the\n lukSF-PV<\/jats:italic>\n ; however, data describing their prevalence and distribution in the Nigerian setting are sparse in the literature, and thus informed the need for the current study.\n <\/jats:p>\n <\/jats:sec>\n \n Objective:<\/jats:title>\n \n We aimed to detect\n mecA<\/jats:italic>\n and analysed the risk factors associated with\n lukSF-PV<\/jats:italic>\n -producing\n S. aureus<\/jats:italic>\n wound infections.\n <\/jats:p>\n <\/jats:sec>\n \n Design:<\/jats:title>\n This was a single-centre hospital-based descriptive cross-sectional study conducted between March 2019 and September 2019 at the University of Calabar Teaching Hospital, Calabar, Nigeria.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n Aspirates from participants with soft tissue infections were cultured, and all isolates of\n S. aureus<\/jats:italic>\n were tested for the presence of\n lukSF-PV<\/jats:italic>\n using endpoint polymerase chain reaction. The\n mecA<\/jats:italic>\n was also detected, and antibiotic susceptibility testing was performed.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Eighty\n S. aureus<\/jats:italic>\n isolates were identified from 360 participants. Of the eighty, 47 (58.8%) were MRSA and 10 (12.5%) were\n lukSF-PV<\/jats:italic>\n -producing\n S. aureus<\/jats:italic>\n strains. Of the ten, six were MSSA and four were MRSA, but the difference was not statistically significant. A significant association was observed between\n lukSF-PV<\/jats:italic>\n -producing\n S. aureus-<\/jats:italic>\n infected wounds and recurrent skin infections (\n p<\/jats:italic>\n \u2009=\u20090.024), as well as working in a day care nursery home (\n p<\/jats:italic>\n \u2009=\u20090.0008). The majority of\n S. aureus<\/jats:italic>\n isolates were susceptible to tigecycline (76%) and vancomycin (76%), followed by susceptibility to linezolid (72.5%), quinupristin\/dalfopristin (67.2%), levofloxacin (38.6%) and erythromycin (11.7%).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n The prevalence of PVL-positive\n S. aureus<\/jats:italic>\n strains causing soft tissue infections in our setting is seemingly high. There is a need for active surveillance of this gene in patients presenting with\n S. aureus<\/jats:italic>\n soft tissue infections in our setting, ensure antibiotic susceptibility testing, evaluate the impact of these strains on clinical outcomes and prevent the spread of\n lukSF-PV<\/jats:italic>\n -positive\n S. aureus<\/jats:italic>\n strains.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/20499361251357394","type":"journal-article","created":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T13:15:16Z","timestamp":1753017316000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular detection of\n mecA<\/i>\n and\n lukSF-PV<\/i>\n in patients with\n Staphylococcus aureus<\/i>\n soft tissue infections in a tertiary hospital setting, Calabar, Nigeria: a cross-sectional study"],"prefix":"10.1177","volume":"12","author":[{"given":"Christian J.","family":"Ide","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, University of Calabar Teaching Hospital, Calabar, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Godwin I.","family":"Ogban","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, University of Calabar Teaching Hospital, Calabar, Nigeria"},{"name":"Department of Medical Microbiology and Parasitology, College of Medical Sciences, University of Calabar, Calabar, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0509-2480","authenticated-orcid":false,"given":"Bassey E.","family":"Ekeng","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, University of Calabar Teaching Hospital, P.M.B. 1278, Calabar, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ubleni E.","family":"Emanghe","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, University of Calabar Teaching Hospital, Calabar, Nigeria"},{"name":"Department of Medical Microbiology and Parasitology, College of Medical Sciences, University of Calabar, Calabar, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asukwo E.","family":"Onukak","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Uyo, Uyo, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony A.","family":"Iwuafor","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, University of Calabar Teaching Hospital, Calabar, Nigeria"},{"name":"Department of Medical Microbiology and Parasitology, College of Medical Sciences, University of Calabar, Calabar, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7673-2414","authenticated-orcid":false,"given":"Ubong A.","family":"Udoh","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, University of Calabar Teaching Hospital, Calabar, Nigeria"},{"name":"Department of Medical Microbiology and Parasitology, College of Medical Sciences, University of Calabar, Calabar, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella T.","family":"Chukwuma","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, College of Medicine, Enugu State University of Science and Technology, Enugu, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel M.","family":"Jimmy","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Calabar Teaching Hospital, Calabar, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ido E.","family":"Ukpeh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medical Sciences, University of Calabar, Calabar, Nigeria"},{"name":"Department of Internal Medicine, University of Calabar Teaching Hospital, Calabar, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatfeng Y.","family":"Mirabeau","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science, College of Health Sciences, Niger Delta University, Amassoma, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Z.","family":"Egah","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, University of Jos, Jos, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,7,20]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.1878688"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00134-14"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2018.33"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S272733"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1067284"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/533591"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00170"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/ced.15392"},{"issue":"2","key":"e_1_3_4_10_2","first-page":"263","article-title":"Prevalence and genotypic characteristics of Panton-Valentine Leukocidin-producing Staphylococcus aureus isolates obtained in 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