{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:27:42Z","timestamp":1783556862670,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["UEG Journal"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Because of its anti\u2010inflammatory, anti\u2010fibrotic, anti\u2010apoptotic and anti\u2010neoplastic properties, the PPAR\u2010\u03b3 agonist rosiglitazone is an interesting drug for investigating for use in the prevention and treatment of radiation\u2010induced intestinal damage. We aimed to evaluate the radioprotective effect of rosiglitazone in a murine model of acute intestinal damage, assessing whether radioprotection is selective for normal tissues or also occurs in tumour cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice were total\u2010body irradiated (12\u2009Gy), with or without rosiglitazone (5\u2009mg\/kg\/day). After 24 and 72 hours, mice were sacrificed and the jejunum was collected. HT\u201029 human colon cancer cells were irradiated with a single dose of 2 (1000 cells), 4 (1500 cells) or 6 (2000 cells) Gy, with or without adding rosiglitazone (20\u2009\u00b5M) 1 hour before irradiation. HT\u201029\u2010xenografted CD1\u00a0mice were irradiated (16\u2009Gy) with or without rosiglitazone; tumour volumes were measured for 33\u00a0days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Rosiglitazone markedly reduced histological signs of altered bowel structures, that is, villi shortening, submucosal thickening, necrotic changes in crypts, oedema, apoptosis, and inflammatory infiltrate induced by irradiation. Rosiglitazone significantly decreased p\u2010NF\u2010kB p65\u00a0phosphorylation and TGF\u03b2 protein expression at 24 and 72 hours post\u2010irradiation and significantly decreased gene expression of <jats:italic>Collagen1<\/jats:italic>, <jats:italic>Mmp13<\/jats:italic>, <jats:italic>Tnf\u03b1<\/jats:italic> and <jats:italic>Bax<\/jats:italic> at 24 hours and <jats:italic>p53<\/jats:italic> at 72 hours post\u2010irradiation. Rosiglitazone reduced HT\u201029 clonogenic survival, but only produced a slight reduction of xenograft tumour growth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Rosiglitazone exerts a protective effect on normal tissues and reduces alterations in bowel structures and inflammation in a radiation\u2010induced bowel toxicity model, without interfering with the radiation effect on HT\u201029 cancer cells. PPAR\u2010\u03b3 agonists should be further investigated for their application in abdominal and pelvic irradiation.<\/jats:p><\/jats:sec>","DOI":"10.1177\/2050640616640443","type":"journal-article","created":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T02:53:40Z","timestamp":1458096820000},"page":"218-226","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":36,"title":["A PPAR\u2010gamma agonist protects from radiation\u2010induced intestinal toxicity"],"prefix":"10.1002","volume":"5","author":[{"given":"Monica","family":"Mangoni","sequence":"first","affiliation":[{"name":"Radiotherapy Unit Department of Experimental and Clinical Biomedical Sciences University of Florence Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariangela","family":"Sottili","sequence":"additional","affiliation":[{"name":"Radiotherapy Unit Department of Experimental and Clinical Biomedical Sciences 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