{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T01:09:17Z","timestamp":1789002557834,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["UEG Journal"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Predicting the response of inflammatory bowel disease (IBD) patients to infliximab (IFX) is an unmet clinical need. The expression and density of transmembrane tumor necrosis factor\u2010\u03b1 in circulating leukocytes maybe directly related to response by promoting apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>We tested the hypothesis that direct apoptosis assessment by real\u2010time polymerase chain reaction evaluation of pro\u2010apoptotic (Bax) and anti\u2010apoptotic (Bcl\u20102) proteins in peripheral blood mononuclear cells (PBMCs) might be associated with response to IFX.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>IFX na\u00efve patients (Crohn's disease, 32 and ulcerative colitis, 20; 35 responders and 17 non\u2010responders) were evaluated for Bax and Bcl\u20102 mRNA expression levels before and 2 weeks after the first infusion. In a subset of patients, apoptosis was also evaluated using flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After the first infusion, Bax increased more in responders than in non\u2010responders (0.7\u00b1\u20090.38 vs 0.81\u2009\u00b1\u20090.32 and 0.86\u2009\u00b1\u20090.37 vs 0.87\u2009\u00b1\u20090.45, respectively, <jats:italic>p<\/jats:italic>\u2009=\u20090.071). Bcl\u20102 decreased more in responders than in non\u2010responders (0.71\u2009\u00b1\u20090.12 vs 0.63\u2009\u00b1\u20090.13 and 0.81\u2009\u00b1\u20090.28 vs 0.77\u2009\u00b1\u20090.27, respectively, <jats:italic>p<\/jats:italic>\u2009=\u20090.038). The Bax\/Bcl\u20102 ratio increased more in responders than in non\u2010responders (0.99\u2009\u00b1\u20090.5 vs 1.3\u2009\u00b1\u20090.51 and 1.03\u2009\u00b1\u20090.17 vs 1.1\u2009\u00b1\u20090.28, respectively, <jats:italic>p<\/jats:italic>\u2009=\u20090.005). The Bax\/Bcl\u20102 ratio was able to predict response in 33\/52 patients and was correlated to flow cytometry\u2010assessed apoptosis (<jats:italic>r<\/jats:italic>\u2009=\u20090.911; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>An increased Bax\/Bcl\u20102 ratio in PBMCs was associated with therapeutic response to IFX in IBD patients.<\/jats:p><\/jats:sec>","DOI":"10.1177\/2050640618774637","type":"journal-article","created":{"date-parts":[[2018,5,3]],"date-time":"2018-05-03T05:33:50Z","timestamp":1525325630000},"page":"1074-1081","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":27,"title":["An increased Bax\/Bcl\u20102 ratio in circulating inflammatory cells predicts primary response to infliximab in inflammatory bowel disease patients"],"prefix":"10.1002","volume":"6","author":[{"given":"Hamid Asadzadeh","family":"Aghdaei","sequence":"first","affiliation":[{"name":"Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center Research Institute for Gastroenterology and Liver Diseases Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azade Amini","family":"Kadijani","sequence":"additional","affiliation":[{"name":"Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center Research Institute for Gastroenterology and Liver Diseases Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Sorrentino","sequence":"additional","affiliation":[{"name":"IBD Center<!--<institution-id institution-id-type=\"Ringgold\">246010<\/institution-id>--> Virginia Tech Carilion School of Medicine Roanoke VA USA"},{"name":"Department of Clinical and Experimental Medical Sciences University of Udine School of 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