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This study explores the prevalence of muscular lower and upper back pain and stress and associations with physical activity and organisational work support among 77 police investigators. The police investigative interviewers reported high levels of physical activity. Compared to other police employees, they reported similar levels of musculoskeletal back pain, higher levels of upper back pain, and higher levels of stress. Physical activity was not related to musculoskeletal back pain. In the regression analysis, musculoskeletal back pain was negatively associated with organisational work support. Limitations due to low statistical power and a cross-sectional design apply. 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