{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:16:51Z","timestamp":1790435811405,"version":"4.1.0"},"reference-count":10,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Multiple Sclerosis Journal - Experimental, Translational and Clinical"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p> Determine the utility of aquaporin 4 IgG (AQP4-IgG) testing (live cell-based assay) for Neuromyelitis Optica Spectrum Disorders (NMOSD). <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p> We included Mayo Clinic patients (1\/1\/2018-12\/31\/2019) tested for serum AQP4-IgG by live cell-based flow-cytometric assay. Medical records were reviewed to assess if patients fulfilled 2015 NMOSD criteria. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> Of 1371 patients tested, 41 were positive (3%) and all fulfilled NMOSD criteria with AQP4-IgG (specificity\u2009=\u2009100%). Only 10\/1330 testing negative met NMOSD criteria without AQP4-IgG (sensitivity\u2009=\u200980%) and seven of these 10 were MOG-IgG positive. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p> AQP4-IgG by live cell-based assay was highly specific and without false positives in a high throughput setting. <\/jats:p><\/jats:sec>","DOI":"10.1177\/20552173211052656","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T13:49:16Z","timestamp":1637934556000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":31,"title":["Diagnostic value of aquaporin-4-IgG live cell based assay in neuromyelitis optica spectrum disorders"],"prefix":"10.1177","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6400-7760","authenticated-orcid":false,"given":"Vyanka","family":"Redenbaugh","sequence":"first","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayra","family":"Montalvo","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4698-663X","authenticated-orcid":false,"given":"Elia","family":"Sechi","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"},{"name":"Department of Medical, Surgical and Experimental Sciences, University of Sassari, Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Buciuc","sequence":"additional","affiliation":[{"name":"Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Fryer","sequence":"additional","affiliation":[{"name":"Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"McKeon","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"},{"name":"Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanda A.","family":"Lennon","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"},{"name":"Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA"},{"name":"Immunology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Mills","sequence":"additional","affiliation":[{"name":"Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian G.","family":"Weinshenker","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean M.","family":"Wingerchuk","sequence":"additional","affiliation":[{"name":"Department of Neurology, Mayo Clinic College of Medicine, Scottsdale, AZ, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Chen","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"},{"name":"Ophthalmology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Tariq Bhatti","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"},{"name":"Ophthalmology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5653-5600","authenticated-orcid":false,"given":"A. Sebastian","family":"Lopez Chiriboga","sequence":"additional","affiliation":[{"name":"Department of Neurology, Mayo Clinic College of Medicine, Jacksonville, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8387-6491","authenticated-orcid":false,"given":"Sean J.","family":"Pittock","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"},{"name":"Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6661-2910","authenticated-orcid":false,"given":"Eoin P.","family":"Flanagan","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA"},{"name":"Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,11,26]]},"reference":[{"key":"bibr1-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000011"},{"key":"bibr2-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2015-312601"},{"key":"bibr3-20552173211052656","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.01028"},{"key":"bibr4-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12084"},{"key":"bibr5-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2021.0912"},{"key":"bibr6-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000001729"},{"key":"bibr7-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1016\/j.msard.2021.103030"},{"key":"bibr8-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2020.2743"},{"key":"bibr9-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3182a6cb5c"},{"key":"bibr10-20552173211052656","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000231"}],"container-title":["Multiple Sclerosis Journal - Experimental, Translational and Clinical"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/20552173211052656","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/20552173211052656","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/20552173211052656","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T23:59:24Z","timestamp":1740873564000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/20552173211052656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10]]},"references-count":10,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1177\/20552173211052656"],"URL":"https:\/\/doi.org\/10.1177\/20552173211052656","relation":{},"ISSN":["2055-2173","2055-2173"],"issn-type":[{"value":"2055-2173","type":"print"},{"value":"2055-2173","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10]]},"article-number":"20552173211052656"}}