{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:36:13Z","timestamp":1777851373049,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[1973,3,1]],"date-time":"1973-03-01T00:00:00Z","timestamp":99792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[1973,3]]},"abstract":"<jats:p>\n                    Condensation with glyoxylic acid vapor, recently introduced as a highly sensitive method for fluorescence histochemical visualization of biogenic monamines, has been found to allow also the demonstration of certain tryptophan- or dopa-containing peptides. Thus, in model experiments, treatment with glyoxylic acid vapor induced fluorescence from di- and tetrapeptides with tryptophan or dopa in NH\n                    <jats:sub>2<\/jats:sub>\n                    -terminal or COOH-terminal position. Peptides without tryptophan or dopa in terminal positions gave no observable fluorescence. Differences were recorded between peptides with NH\n                    <jats:sub>2<\/jats:sub>\n                    -terminal and COOH-terminal tryptophan with respect to their fluorescence yields under different reaction conditions and the spectral characteristics of the fluorophores (excitation\/emission maxima: 375-380\/500-520 nm and 340-370\/435 nm, respectively). In addition, the tryptophan-containing peptides could be distinguished microspectrofluorometrically from the dopa-containing peptides. Thus, the fluorophore of the NH\n                    <jats:sub>2<\/jats:sub>\n                    -terminal dopa peptide had excitation\/emission maxima at 330 and 380\/495 nm, and the corresponding values for the fluorophore of the COOH-terminal dopa peptide were 330 and 370\/460 nm. The glyoxylic acid treatment produced intense fluorescence in certain endocrine cell systems in pituitary, thyroid and gastric mucosa, previously suggested to store peptides with NH\n                    <jats:sub>2<\/jats:sub>\n                    -terminal tryptophan. The present findings support this hypothesis.\n                  <\/jats:p>","DOI":"10.1177\/21.3.253","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T01:19:10Z","timestamp":1301620750000},"page":"253-265","source":"Crossref","is-referenced-by-count":20,"title":["FLUORESCENCE HISTOCHEMICAL DEMONSTRATION OF PEPTIDES WITH NH\n                    <sub>2<\/sub>\n                    - OR COOH-TERMINAL TRYPTOPHAN OR DOPA BY CONDENSATION WITH GLYOXYLIC ACID"],"prefix":"10.1177","volume":"21","author":[{"given":"ANDERS","family":"BJ\u00d6RKLUND","sequence":"first","affiliation":[{"name":"Departments of Histology and Pharmacology, University of Lund, Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ROLF","family":"HAKANSON","sequence":"additional","affiliation":[{"name":"Departments of Histology and Pharmacology, University of Lund, Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"OLLE","family":"LINDVALL","sequence":"additional","affiliation":[{"name":"Departments of Histology and Pharmacology, University of Lund, Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FRANK","family":"SUNDLER","sequence":"additional","affiliation":[{"name":"Departments of Histology and Pharmacology, University of Lund, Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1973,3,1]]},"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/21.3.253","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/21.3.253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:20:22Z","timestamp":1777501222000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/21.3.253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1973,3]]}},"alternative-id":["10.1177\/21.3.253"],"URL":"https:\/\/doi.org\/10.1177\/21.3.253","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,3]]}}}