{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T04:10:31Z","timestamp":1788667831426,"version":"build-2803163510"},"reference-count":16,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"The Longevity Cell Bank, Biothrive International Group, Muscat, Oman"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cellular Reprogramming"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>\n                    The low efficiency in the production of induced pluripotent stem cell (iPSC) remains a major barrier to the widespread reprogramming applications in regenerative medicine and drug screening. Small molecules that modulate key signaling pathways offer a promising approach to overcome the reprogramming limitation. Here, we demonstrated that Thiazovivin (TZV), a selective Rho-associated coiled-coil kinase inhibitor, significantly increased human fibroblast reprogramming rate by approximately 1.4-fold (*\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.01). Treatment with TZV during the early phase of reprogramming enhanced colony formation, improved cell survival, and promoted the mesenchymal-to-epithelial transition (MET). Quantitative Real Time Polymerase Chain Reaction (RT-PCR) analysis revealed the downregulation of mesenchymal markers (N-cadherin, 52%; vimentin, 61%;\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.05) and upregulation of epithelial markers (E-cadherin, 165%; EpCAM, 95%;\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.05). Flow cytometry confirmed elevated expression of NANOG and TRA-1-60 pluripotency markers. Collectively, these findings suggest that TZV facilitates reprogramming through MET, offering a simple and effective strategy to enhance the efficiency and scalability of human iPSC generation.\n                  <\/jats:p>","DOI":"10.1177\/21524971261484998","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T08:55:23Z","timestamp":1788166523000},"page":"137-142","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["ROCK Inhibition Accelerates Mesenchymal-to-Epithelial Transition and Improves Early Survival During Human Fibroblast Reprogramming"],"prefix":"10.1177","volume":"28","author":[{"given":"Mahsa","family":"Azhdari","sequence":"first","affiliation":[{"name":"Biochemistry Department, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samaneh","family":"Dehghan","sequence":"additional","affiliation":[{"name":"Eye Research Center, The Five Senses Health Institute, Moheb Kowsar Hospital, Iran University of Medical Sciences, Tehran, Iran."},{"name":"Stem Cells and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Ghamari","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Mirshahi","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5502-6457","authenticated-orcid":false,"given":"Alireza","family":"Shoae-Hassani","sequence":"additional","affiliation":[{"name":"Eye Research Center, The Five Senses Health Institute, Moheb Kowsar Hospital, Iran University of Medical Sciences, Tehran, Iran."},{"name":"Department of Neurosurgery, University of Pennsylvania, Philadelphia, Pennsylvania, USA."},{"name":"The Longevity Cell Bank, Biothrive International Group, Muscat, Oman."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.05083.x"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12202499"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11030430"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13090780"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2025.01.006"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.04.014"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201806065"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-60717-z"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10411"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2025.1538806"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.08.003"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0195-z"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019102961"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092078"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1310"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002024107"}],"container-title":["Cellular Reprogramming"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/21524971261484998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/21524971261484998","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/21524971261484998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T03:23:48Z","timestamp":1788665028000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/21524971261484998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10,1]]}},"alternative-id":["10.1177\/21524971261484998"],"URL":"https:\/\/doi.org\/10.1177\/21524971261484998","relation":{},"ISSN":["2152-4971","2152-4998"],"issn-type":[{"value":"2152-4971","type":"print"},{"value":"2152-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]}}}