{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T07:18:48Z","timestamp":1777879128311,"version":"3.51.4"},"reference-count":27,"publisher":"SAGE Publications","issue":"1-2","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP25am0521010 (to Y.K.), JP25ama121008 (to Y.K.),"],"award-info":[{"award-number":["JP25am0521010 (to Y.K.), JP25ama121008 (to Y.K.),"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>\n                    Ephrin type-B receptor 3 (EphB3) binds to transmembrane ephrin-B ligands to regulate cell migration, adhesion, and proliferation. EphB3 exhibits a gradient expression pattern in the normal intestine, with the highest levels at the crypt base, and plays a crucial role in the maintenance of normal intestinal epithelium. Therefore, anti-EphB3 monoclonal antibodies (mAbs) are required for basic research and diagnosis. In this study, we developed novel antihuman EphB3, Eb\n                    <jats:sub>3<\/jats:sub>\n                    Mab-5 (IgG\n                    <jats:sub>1<\/jats:sub>\n                    , \u03ba) and Eb\n                    <jats:sub>3<\/jats:sub>\n                    Mab-11 (IgG\n                    <jats:sub>1<\/jats:sub>\n                    , \u03ba), using the Cell-Based Immunization and Screening (CBIS) method. Eb\n                    <jats:sub>3<\/jats:sub>\n                    Mab-5 and Eb\n                    <jats:sub>3<\/jats:sub>\n                    Mab-11 reacted with EphB3-overexpressed Chinese hamster ovary-K1 (CHO\/EphB3) and endogenous EphB3-positive colorectal cancer LS174T in flow cytometry. The apparent binding affinity of Eb\n                    <jats:sub>3<\/jats:sub>\n                    Mab-5 for CHO\/EphB3 and LS174T was 7.6 \u00d7 10\n                    <jats:sup>\u22129<\/jats:sup>\n                    M and 1.7 \u00d7 10\n                    <jats:sup>\u22128<\/jats:sup>\n                    M, respectively. Eb\n                    <jats:sub>3<\/jats:sub>\n                    Mab-11 could detect EphB3 in western blot analysis and immunohistochemistry. Eb\n                    <jats:sub>3<\/jats:sub>\n                    Mab-5 and Eb\n                    <jats:sub>3<\/jats:sub>\n                    Mab-11, established by the CBIS method, may contribute to the diagnosis and therapy of EphB3-positive tumors.\n                  <\/jats:p>","DOI":"10.1177\/21679436251408319","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T16:49:46Z","timestamp":1767113386000},"page":"1-10","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of Novel Anti-EphB3 Monoclonal Antibodies for Multiple Applications"],"prefix":"10.1177","volume":"45","author":[{"given":"Guanjie","family":"Li","sequence":"first","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika K.","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukinari","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-023-00634-x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.110556"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.506"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1275330"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11020315"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23052761"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2024.10.002"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(02)01015-2"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0278-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.10.018"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.04.002"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1350108"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.Can-05-3849"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102225"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom10040602"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/352616"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2011.05.044"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.36922\/mi.5728"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2025.101998"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2025.102130"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26178302"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3892\/or.2021.8083"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.70074"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2025.115809"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021702"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.30678"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2009.09.010"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; 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