{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:15:19Z","timestamp":1783124119723,"version":"3.54.6"},"reference-count":46,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T00:00:00Z","timestamp":1779494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP25am0521010 (to Y.K.), JP25ama121008 (to Y.K.),"],"award-info":[{"award-number":["JP25am0521010 (to Y.K.), JP25ama121008 (to Y.K.),"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    Erythropoietin-producing hepatocellular carcinoma (Eph) receptor A4 (EphA4) binds to a broad range of ephrin ligands and is upregulated in various tumors. EphA4 activation plays crucial roles in the maintenance of cancer invasiveness, immune evasion, and stem cell properties. Furthermore, a novel EphA4 ligand, secretory ribonuclease 1, has been identified, and its interaction stimulates EphA4 signaling, leading to the promotion of cancer stem cell properties. Therefore, EphA4 has been considered an attractive target for cancer therapies. Thus, monoclonal antibodies (mAbs) against EphA4 are essential for basic research and mAb-based treatment in the clinic. In this study, we developed a novel anti-human EphA4 mAb, Ea\n                    <jats:sub>4<\/jats:sub>\n                    Mab-3, using the Cell-Based Immunization and Screening (CBIS) method. Ea\n                    <jats:sub>4<\/jats:sub>\n                    Mab-3 reacted with EphA4-overexpressed Chinese hamster ovary-K1 (CHO\/EphA4) and endogenous EphA4-positive lung squamous cell carcinoma LK-2 in flow cytometry. The binding affinities (apparent dissociation constant\n                    <jats:italic toggle=\"yes\">K<\/jats:italic>\n                    <jats:sub>D<\/jats:sub>\n                    values) were determined as 4.5 \u00d7 10\n                    <jats:sup>\u22129<\/jats:sup>\n                    M for CHO\/EphA4 and 2.5 \u00d7 10\n                    <jats:sup>\u22129<\/jats:sup>\n                    M for LK-2. Furthermore, Ea\n                    <jats:sub>4<\/jats:sub>\n                    Mab-3 did not exhibit cross-reactivity with other Eph receptor-overexpressed CHO-K1 cells. In addition, Ea\n                    <jats:sub>4<\/jats:sub>\n                    Mab-3 is suitable for immunoblotting and immunohistochemistry. These results indicate that Ea\n                    <jats:sub>4<\/jats:sub>\n                    Mab-3, established using the CBIS method, facilitates basic studies of EphA4 and is expected to be useful for mAb-based tumor diagnosis and therapy.\n                  <\/jats:p>","DOI":"10.1177\/21679436261450334","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T12:59:07Z","timestamp":1779541147000},"page":"48-57","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of a Novel Anti-EphA4 Monoclonal Antibody, Ea\n                    <sub>4<\/sub>\n                    Mab-3, for Versatile Applications"],"prefix":"10.1177","volume":"45","author":[{"given":"Ayano","family":"Saga","sequence":"first","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanjie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika K.","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5385-8201","authenticated-orcid":false,"given":"Yukinari","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,5,23]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-023-00634-x"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2024.10.002"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a009159"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2007.07.019"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.328"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2867-10-41"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074464"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11020315"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024426"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.Mct-07-2263"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.Mct-12-0030"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2006.00313.x"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.5650"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3041"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.14938"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23075-2"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.36922\/mi.5728"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2025.101960"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2025.101998"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2021.8124"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2017.0042"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2017.0026"},{"key":"e_1_3_2_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10014-015-0222-8"},{"key":"e_1_3_2_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.08.171"},{"key":"e_1_3_2_26_1","doi-asserted-by":"publisher","DOI":"10.36922\/MI025220049"},{"key":"e_1_3_2_27_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21871"},{"key":"e_1_3_2_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01450-2"},{"key":"e_1_3_2_29_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1713137"},{"key":"e_1_3_2_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(23)00221-0"},{"key":"e_1_3_2_31_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2204619"},{"key":"e_1_3_2_32_1","doi-asserted-by":"publisher","DOI":"10.1200\/jco.21.01626"},{"key":"e_1_3_2_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-025-02206-x"},{"key":"e_1_3_2_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2018.02.012"},{"key":"e_1_3_2_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03597-1"},{"key":"e_1_3_2_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.09.030"},{"issue":"10","key":"e_1_3_2_37_1","first-page":"4865","article-title":"Structural 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Structural insights into EphA4 unconventional activation from prediction of the EphA4 and its complex with ribonuclease 1. 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