{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T04:25:41Z","timestamp":1793679941737,"version":"4.5.1"},"reference-count":37,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"2025 Hunan University of Chinese Medicine University-Hospital Joint Fund","award":["(2025XYLH146)"],"award-info":[{"award-number":["(2025XYLH146)"]}],"id":[{"id":"https:\/\/ror.org\/01tsmvz08","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Global Spine Journal"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Translational animal study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the therapeutic effects of needle-knife therapy in cervical spondylotic radiculopathy (CSR) rat models. This study also aims to determine whether these effects are mediated via inositol-requiring enzyme 1 alpha (IRE1\u03b1)-X-box binding protein 1 (XBP1) axis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A CSR rat model was established via spinal canal insertion. The IRE1\u03b1 activator IXA4 was administered to enhance IRE1\u03b1-XBP1 signaling activity. Motor function and pain sensitivity were evaluated using paw withdrawal mechanical threshold (PWMT), paw withdrawal thermal latency (PWTL), and gait scoring. Microglial activation was evaluated by immunofluorescence staining for ionized calcium-binding adapter molecule 1 (Iba1) and tumor necrosis factor-alpha (TNF-\u03b1). Neuronal apoptosis was assessed via TUNEL\/NeuN double immunofluorescence, qRT-PCR, and Western blotting (WB). Key proteins in the IRE1\u03b1-XBP1 signaling were analyzed using WB.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CSR rats exhibited spinal cord inflammation, neuronal apoptosis, and aberrant activation of the IRE1\u03b1-XBP1 pathway. Needle-knife therapy significantly mitigated spinal cord pathology. The treatment suppressed inflammatory cytokines, inhibited pro-inflammatory microglial activation, and inhibited activation of IRE1\u03b1-XBP1 signaling. Administration of IXA4 partially reversed these protective effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Needle-knife therapy ameliorates inflammation and neuronal apoptosis in CSR. These effects are associated with downregulation of the IRE1\u03b1-XBP1 signaling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/21925682261443920","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T22:33:06Z","timestamp":1776378786000},"page":"3794-3806","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Needle-Knife Therapy Relieves Inflammation and Neuronal Apoptosis in Cervical Spondylotic Radiculopathy via IRE1\u03b1-XBP1 Signaling"],"prefix":"10.1177","volume":"16","author":[{"given":"Juyao","family":"Liu","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Hunan College of Traditional Chinese Medicine (Hunan Province Directly Affiliated TCM Hospital)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangsheng","family":"Long","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of 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