{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T03:46:16Z","timestamp":1782618376750,"version":"3.54.5"},"reference-count":74,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"NIH Functional Proteomics of Aging Training Grant","award":["5T32AG029796-13"],"award-info":[{"award-number":["5T32AG029796-13"]}]},{"name":"NIH Functional Proteomics of Aging Training Grant","award":["5T32AG029796-14"],"award-info":[{"award-number":["5T32AG029796-14"]}]},{"name":"National Institutes of Health (NIH) Minnesota Muscle Training Grant","award":["5T32AR007612-21"],"award-info":[{"award-number":["5T32AR007612-21"]}]},{"name":"National Institutes of Health (NIH) Minnesota Muscle Training Grant","award":["5T32AR007612-22"],"award-info":[{"award-number":["5T32AR007612-22"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R01AR042423-30"],"award-info":[{"award-number":["5R01AR042423-30"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R01AR049899-21"],"award-info":[{"award-number":["5R01AR049899-21"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neuromuscular Diseases"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>\n                      Skeletal muscle constitutes 30\u201340% of total body mass and is now considered an endocrine organ, given its secretion of a variety proteins, metabolites, and cytokines. We have previously shown that the absence of dystrophin in skeletal muscle contributes to lethal systemic stress pathology in the\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      mouse model of Duchenne muscular dystrophy through a mechanism that remains to be identified. Here we searched for secreted protein signaling factors, or myokines, released from dystrophin-deficient skeletal muscle that influence the organism-wide integrated stress response. We performed skeletal muscle extracellular fluid extraction and discovery proteomics for wild-type,\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      , and transgenic\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      mice rescued by expression of a dystrophin construct, all analyzed under basal conditions and following brief scruff restraint stress that causes inactivity in\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Major Findings:<\/jats:title>\n                    <jats:p>\n                      Our analysis demonstrated that skeletal muscle dystrophinopathy is associated with increased expression of numerous proteins in both intact\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      skeletal muscle and extracellular fluid compared to healthy mice. Brief scruff restraint revealed protein candidates with differential abundance in\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      extracellular fluid. Specifically, altered follistatin-like 1 protein and adiponectin secretion in response to scruff stress was shown to be dependent on skeletal muscle dystrophinopathy. The diverse signaling roles of follistatin-like 1 in the cardiovascular, musculoskeletal, and nervous system implicate it as a particularly intriguing myokine candidate regulating the\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      stress response.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      Our current study informs on the skeletal muscle secretory profile in\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      following a stressful stimulus and provides new leads to elucidate the mechanism by which\n                      <jats:italic toggle=\"yes\">mdx<\/jats:italic>\n                      skeletal muscle orchestrates inter-organ stress signaling.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/22143602251409302","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T09:02:20Z","timestamp":1766480540000},"page":"698-721","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of myokines associated with the pathological stress response in the\n                    <i>mdx<\/i>\n                    mouse model of Duchenne muscular dystrophy"],"prefix":"10.1177","volume":"13","author":[{"given":"Erynn E","family":"Johnson","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Medical School, Minneapolis, MN, 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Powers","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Medical School, Minneapolis, MN, 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M","family":"Ervasti","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Medical School, Minneapolis, MN, 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,12,23]]},"reference":[{"key":"e_1_3_4_2_2","first-page":"1","article-title":"Duchenne muscular dystrophy","volume":"7","author":"Duan D","year":"2021","unstructured":"Duan D, Goemans N, Takeda S, et al. Duchenne muscular dystrophy. Nat Rev Dis Prim 2021; 7: 1\u201319.","journal-title":"Nat Rev Dis Prim"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000008363"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2007.06.465"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/0883073817701368"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awac048"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31753-3"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-277X.2009.00979.x"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.102700"},{"key":"e_1_3_4_10_2","first-page":"312","article-title":"Retention of stress susceptibility in the mdx mouse model of duchenne muscular dystrophy after PGC-1\u03b1 overexpression or ablation of IDO1 or CD38","volume":"26","author":"Johnson EE","year":"2024","unstructured":"Johnson EE, Southern WM, Doud B, et al. Retention of stress susceptibility in the mdx mouse model of duchenne muscular dystrophy after PGC-1\u03b1 overexpression or ablation of IDO1 or CD38. Hum Mol Genet 2024; 26: 312\u2013318.","journal-title":"Hum Mol Genet"},{"key":"e_1_3_4_11_2","first-page":"4951","article-title":"Independent variability of microtubule perturbations associated with dystrophinopathy","volume":"25","author":"Belanto JJ","year":"2016","unstructured":"Belanto JJ, Olthoff JT, Mader TL, et al. Independent variability of microtubule perturbations associated with dystrophinopathy. Hum Mol Genet 2016; 25: 4951\u20134961.","journal-title":"Hum Mol Genet"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/84.3.475"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ocarto.2019.100019"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2465-5"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3233\/BPL-210133"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1210\/endrev\/bnaa016"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.108175"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.12.014"},{"key":"e_1_3_4_19_2","first-page":"115","article-title":"Principles and problems of exosome isolation from biological fluids","volume":"16","author":"Yakubovich EI","year":"2022","unstructured":"Yakubovich EI, Polischouk AG, Evtushenko VI. Principles and problems of exosome isolation from biological fluids. Biochem (Mosc) Suppl Ser A Membr Cell Biol 2022; 16: 115.","journal-title":"Biochem (Mosc) Suppl Ser A Membr Cell Biol"},{"key":"e_1_3_4_20_2","first-page":"1","article-title":"Potential and challenges of specifically isolating extracellular vesicles from heterogeneous populations","volume":"11","author":"Allelein S","year":"2021","unstructured":"Allelein S, Medina-Perez P, Lopes ALH, et al. Potential and challenges of specifically isolating extracellular vesicles from heterogeneous populations. Sci Reports 2021; 11: 1\u201312.","journal-title":"Sci Reports"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.08.039"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.3109\/13813455.2013.768270"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-018-2805-0"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2022.114060"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010235"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac026117i"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2014.980775"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-48"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz369"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-017-1600-5"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac336"},{"key":"e_1_3_4_32_2","article-title":"Uniprot: the universal protein knowledgebase in 2023","volume":"51","author":"Bateman A","year":"2023","unstructured":"Bateman A, Martin MJ, Orchard S, et al. Uniprot: the universal protein knowledgebase in 2023. Nucleic Acids Res 2023; 51: D523\u2013D531.","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2022.10.300"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv381"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07639-3"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2019.05.008"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201300072"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202317405"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12517"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa7322"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12527"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13023-023-02885-1"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12708"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1248-2"},{"key":"e_1_3_4_45_2","first-page":"94","article-title":"Dynamic resistance exercise increases skeletal muscle-derived FSTL1 inducing cardiac angiogenesis via DIP2A\u2013Smad2\/3 in rats following myocardial infarction","volume":"10","author":"Xi Y","year":"2021","unstructured":"Xi Y, Hao M, Liang Q, et al. Dynamic resistance exercise increases skeletal muscle-derived FSTL1 inducing cardiac angiogenesis via DIP2A\u2013Smad2\/3 in rats following myocardial infarction. J Sport Heal Sci 2021; 10: 94.","journal-title":"J Sport Heal Sci"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15300"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s42978-019-0009-4"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M803440200"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153619"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-014-8526-z"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.26195"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2012.01.010"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2020.09.031"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202002500R"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101730"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.1.115"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2016.05.006"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu366"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13395-015-0051-9"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12071407"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIOX13121551"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00638.2023"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1113\/EP091274"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.3390\/CELLS12162101"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12531"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-018-0501-z"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42203-z"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2023.10.010"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.10.404"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aay8416"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2015.06.238"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3600001"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2006.01.006"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIOX10121876"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1011"}],"container-title":["Journal of Neuromuscular Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/22143602251409302","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/22143602251409302","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/22143602251409302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T03:36:16Z","timestamp":1782617776000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/22143602251409302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,23]]},"references-count":74,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1177\/22143602251409302"],"URL":"https:\/\/doi.org\/10.1177\/22143602251409302","relation":{},"ISSN":["2214-3599","2214-3602"],"issn-type":[{"value":"2214-3599","type":"print"},{"value":"2214-3602","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,23]]}}}