{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T02:49:43Z","timestamp":1778726983339,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"},{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Orthop Surg (Hong Kong)"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose.<\/jats:title>\n                    <jats:p>To investigate the efficacy of local biodegradable composites of hydroxyapatite, plaster of Paris, and a binder of either alginate or chitosan impregnated with methotrexate on human mammary carcinoma cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods.<\/jats:title>\n                    <jats:p>An in vitro analysis of drug dissolution and a cytotoxicity test on human mammary carcinoma cells were performed over one month. Physicochemical properties of each composite were investigated using scanning electron microscopy, X-ray diffractometry, and Fourier transform infrared spectroscopy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results.<\/jats:title>\n                    <jats:p>Both composites with a binder of either alginate or chitosan could release methotrexate for over one month. The amount of methotrexate released depended on the amount of methotrexate loaded. The composite using alginate as a binder released a significantly greater amount of methotrexate than that using chitosan as a binder (p&lt;0.05). The elution of both composites showed favourable cytotoxicity when the concentration was greater than 5 \u03bcg\/ml.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion.<\/jats:title>\n                    <jats:p>Methotrexate-impregnated hydroxyapatite composites appear to be effective local skeletal methotrexate delivery systems against human mammary carcinoma cells in an in vitro model.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/230949900701500113","type":"journal-article","created":{"date-parts":[[2016,10,4]],"date-time":"2016-10-04T17:13:55Z","timestamp":1475601235000},"page":"56-61","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["The Efficacy of Methotrexate-Impregnated Hydroxyapatite Composites on Human Mammary Carcinoma Cells"],"prefix":"10.1177","volume":"15","author":[{"given":"J","family":"Vechasilp","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery and Physical Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Tangtrakulwanich","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery and Physical Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Oungbho","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Prince of Songkla University, Hat Yai, Songkhla, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Yuenyongsawad","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Botany, Prince of Songkla University, Hat Yai, Songkhla, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2007,4,1]]},"reference":[{"key":"bibr1-230949900701500113","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-198706000-00038"},{"key":"bibr2-230949900701500113","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5173(95)00059-R"},{"key":"bibr3-230949900701500113","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19980315)39:4<536::AID-JBM5>3.0.CO;2-K"},{"key":"bibr4-230949900701500113","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.81B3.9167"},{"key":"bibr5-230949900701500113","doi-asserted-by":"publisher","DOI":"10.3928\/0147-7447-19920201-13"},{"key":"bibr6-230949900701500113","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2000.18.6.1378"},{"key":"bibr7-230949900701500113","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(94)90272-0"},{"key":"bibr8-230949900701500113","doi-asserted-by":"crossref","unstructured":"Sarid D. 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