{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T08:18:26Z","timestamp":1793089106558,"version":"4.5.0"},"reference-count":40,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T00:00:00Z","timestamp":1595203200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000072","name":"national institute of dental and craniofacial research","doi-asserted-by":"publisher","award":["DE14368"],"award-info":[{"award-number":["DE14368"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["JDR Clinical &amp; Translational Research"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To compare the oral microbiota of Sj\u00f6gren\u2019s syndrome (SS) with that of healthy subjects (HS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Supragingival and subgingival biofilm samples were collected from the mesial-buccal tooth surfaces of SS patients (n = 57) and age- and sex-matched HS (n = 53). Unstimulated saliva and 8 oral tissue samples were taken using a buccal brush. Caries and periodontal measures were recorded. All supragingival samples and a subgroup of 24 SS and 28 HS subgingival samples, as well as 32 SS and 11 HS saliva and oral tissue samples, were analyzed for their content of 41 bacterial species using checkerboard DNA-DNA hybridization. Mean levels (\u00d710\n                      <jats:sup>5<\/jats:sup>\n                      \u00b1 SEM) and percentage of DNA probe counts of each species were determined for each sample site and averaged within subjects in the 2 clinical groups. Kruskal-Wallis tests, adjusting for multiple comparisons and cluster analysis, were used for soft tissue and microbial analysis, and the Mann-Whitney test was used to compare caries and periodontal measures.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Mean (\u00d710\n                      <jats:sup>5<\/jats:sup>\n                      \u00b1 SEM) total DNA probe counts in supragingival samples were significantly lower (P &lt; 0.001) in the SS (13.3 \u00b1 .7) compared to the HS (44.1 \u00b1 6.8) group. In supragingival samples, Veillonella parvula, Fusobacterium nucleatum ss vincenti, and Propionibacterium acnes were markedly elevated in the SS compared to the HS group in both mean (\u00d710\n                      <jats:sup>5<\/jats:sup>\n                      \u00b1 SEM) and mean (\u00b1 SEM) percentage DNA probe counts (P &lt; 0.001). In subgingival samples of SS, V. parvula was significantly different compared to HS (P &lt; 0.05). SS was characterized by high levels of purple and low levels of orange and red complexes. Cluster analysis of oral tissues and saliva demonstrated that the mean microbial profiles for SS patients and the HS group clustered separately. Active root caries (P &lt; 0.003) and attachment loss were significantly higher (P &lt; 0.029) in the SS group compared to the HS group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>These findings indicate that saliva is a major controlling factor of intraoral biofilm. V. parvula may be a unique microbial biomarker for Sj\u00f6gren\u2019s syndrome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Knowledge Transfer Statement<\/jats:title>\n                    <jats:p>The microbiome characterized for Sj\u00f6gren\u2019s syndrome in salivary hypofunction is shown to be under stress and reduced. Veillonella parvula can be a possible identification of a biomarker for Sj\u00f6gren\u2019s syndrome.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/2380084420940623","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T01:14:16Z","timestamp":1595294056000},"page":"324-332","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Characterizing Microbiota from Sj\u00f6gren\u2019s Syndrome Patients"],"prefix":"10.1177","volume":"6","author":[{"given":"M.","family":"Singh","sequence":"first","affiliation":[{"name":"Division of Oral Medicine, School of Dental Medicine, Tufts University, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Teles","sequence":"additional","affiliation":[{"name":"Department of Basic and Translational Sciences, University of Pennsylvania, School of Dental Medicine, Philadelphia, PA, USA"},{"name":"Center for Innovation & Precision Dentistry, School of Engineering and Applied Sciences, University of Pennsylvania, School of Dental Medicine, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.G.","family":"Uzel","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dental Medicine, Tufts University, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Papas","sequence":"additional","affiliation":[{"name":"Division of Oral Medicine, School of Dental Medicine, Tufts University, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,7,20]]},"reference":[{"key":"bibr1-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1177\/00220345990780080601"},{"key":"bibr2-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.011"},{"issue":"3","key":"bibr3-2380084420940623","first-page":"839","volume":"31","author":"Bhatti MA","year":"2000","journal-title":"Veillonella parvula"},{"key":"bibr4-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1177\/00220345870660S107"},{"key":"bibr5-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.39.100185.001135"},{"key":"bibr6-2380084420940623","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2008.0101"},{"key":"bibr7-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MBP-0013-2014"},{"key":"bibr8-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.2016.865"},{"key":"bibr9-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-007-0132-1"},{"key":"bibr10-2380084420940623","doi-asserted-by":"publisher","DOI":"10.3390\/s120302519"},{"key":"bibr11-2380084420940623","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.5109"},{"key":"bibr12-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2007.00409.x"},{"key":"bibr13-2380084420940623","first-page":"S225","volume":"37","author":"Maarse F","year":"2019","journal-title":"Clin Exp Rheumatol"},{"key":"bibr14-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2003.00376.x"},{"key":"bibr15-2380084420940623","doi-asserted-by":"crossref","unstructured":"Mandl T, Marsal J, Olsson P, Ohlsson B, Andr\u00e9asson K. 2017. Severe intestinal dysbiosis is prevalent in primary Sj\u00f6gren\u2019s syndrome and is associated with systemic disease activity Arthritis Res Ther. 19(1):237.","DOI":"10.1186\/s13075-017-1446-2"},{"key":"bibr16-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12098"},{"key":"bibr17-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(97)90144-X"},{"key":"bibr18-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1128\/jb.105.3.999-1005.1971"},{"key":"bibr19-2380084420940623","first-page":"2149","author":"Niazi SA","year":"2016","journal-title":"Propionibacterium acnes"},{"key":"bibr20-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12589"},{"key":"bibr21-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1038\/srep23561"},{"key":"bibr22-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6890-5-4"},{"key":"bibr23-2380084420940623","first-page":"6","author":"Pitts NB","year":"1997","journal-title":"Community Dent Health"},{"key":"bibr24-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-02900-1"},{"key":"bibr25-2380084420940623","first-page":"953","volume":"74","author":"Pulukool S","year":"2015","journal-title":"Ann Rheum Dis"},{"key":"bibr26-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1586\/epr.11.80"},{"key":"bibr27-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0218319"},{"key":"bibr28-2380084420940623","volume-title":"Oral and maxillofacial medicine: the basis of diagnosis and treatment","author":"Scully C","year":"2008","edition":"2"},{"key":"bibr29-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2019.1660566"},{"key":"bibr30-2380084420940623","doi-asserted-by":"publisher","DOI":"10.3402\/jom.v8.31119"},{"key":"bibr31-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00107.x"},{"key":"bibr32-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"bibr33-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1991.tb00070.x"},{"issue":"4","key":"bibr34-2380084420940623","first-page":"788","volume":"17","author":"Socransky SS","year":"1994","journal-title":"Biotechniques"},{"key":"bibr35-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2014.02.004"},{"key":"bibr36-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2003.00015.x"},{"key":"bibr37-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.6.554"},{"key":"bibr38-2380084420940623","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.8.3158-3165.1995"},{"key":"bibr39-2380084420940623","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.5649"},{"key":"bibr40-2380084420940623","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.54.61"}],"container-title":["JDR Clinical &amp; Translational Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/2380084420940623","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/2380084420940623","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/2380084420940623","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:44:55Z","timestamp":1777495495000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/2380084420940623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,20]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1177\/2380084420940623"],"URL":"https:\/\/doi.org\/10.1177\/2380084420940623","relation":{},"ISSN":["2380-0844","2380-0852"],"issn-type":[{"value":"2380-0844","type":"print"},{"value":"2380-0852","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,20]]}}}